WPRIM Management System> DCMS> Chinese Journal of Biotechnology> 2006> 22

Volume: 22

1. The analysis of heterogeneity of HWTX-I expressed in Pichia pastoris. Page:215—219
2. Introduce Tagsk1 into salt-sensitive callus to improve the capacity of salt-tolerance by micropartical bombardment. Page:211—214
3. Generation of selectable marker-free and vector backbone sequence-free Xa21 transgenic rice. Page:204—210
4. The changes of rare codon and mRNA structure accelerate expression of qa-3 in Escherichia coli. Page:198—203
5. Dual promoters enhance heterologous enzyme production from bacterial phage based recombinant Bacillus subtilis. Page:191—197
6. High-level expression of phenylalanine ammonia-lyase in Lactococcus Lactis via synthesized sequence based on bias codons. Page:187—190
7. Use of Ssp dnaB mini-intein as a fusion partner for preparation of recombinant human brain natriuretic peptide. Page:180—203 passim
8. Expression in Pichia pastoris and properties of human serum albumin-interferon alpha2b chimera. Page:173—179
9. Application of microfluidic-chip in biomedicine. Page:167—171
10. The effect of glutamine on the growth, metabolism and endostatin production of microencapsulated rCHO cells. Page:162—166
11. Effect of osmotic pressure on nitrification. Page:156—161
12. Preparation of uniform-sized chitosan microspheres and application as carriers for protein drugs. Page:150—155
13. Modelling a penicillin fed-batch fermentation using least squares support vector machines. Page:144—149
14. Stimulatory effects of some amino acids on glycerol production by Candida glycerinogenes. Page:138—143
15. Construction of sugar-based microbial fuel cells by dissimilatory metal reduction bacteria. Page:131—137
16. Screening and detecting of proteins interacting with mPem. Page:125—130
17. Studies on phage displayed mimotopes of a protective monoclonal antibody (SSj14) against Schistosoma japonicum. Page:119—124
18. Immobilization of lipase on macroporous resin and its application in synthesis of biodiesel in low aqueous media. Page:114—118
19. Hairy root induction and plant regeneration of crownvetch (Coronilla varia L.) transformed by Agrobacterium rhizogenes. Page:107—113
20. Effects of hydrodynamic on aggregates formation, growth and metabolism of HEK293 cells in suspension culture. Page:101—106
21. Metabolism of recombinant CHO-GS cell reducing of toxic effect of ammonia. Page:94—100
22. High level expression, purification and cytotoxicity of IL-10(18-57)-PE40. Page:87—93
23. Recombinant Aspergillus niger glucose oxidase expressed in Trichoderma reesei. Page:82—86
24. Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis. Page:77—81
25. High-level production of neutral endoglucanase 1 in Pichia pastoris. Page:71—76
26. Acute toxicity and immunoprotection of recombinant apxI toxin of Actinobacillus pleuropneumoniae in mice. Page:65—70
27. Prokaryotic expression of functional PTEN in Escherichia coli and preparation of polyclonal antibody. Page:58—64
28. Molecular cloning of tomato LeHsp110/ClpB gene and its effect on the thermotolerance in plant. Page:52—57
29. Cloning, soluble expression and characterization of human sBCMA. Page:46—51
30. Study on immunogenicity of the N-terminal polypeptide of RTX toxin I of Actinobacillus pleuropneumoniae. Page:39—45
31. Functional expression of an omega-3 fatty acid desaturase gene from Glycine max in Saccharomyces cerevisiae. Page:33—38
32. Improvement of the thermostability of xylanase by N-terminus replacement. Page:26—32
33. Large recombinant protein displayed on filamentous phage surface and its interaction with small molecule. Page:19—25
34. Development and challenge of modified hemoglobins as red blood cell substitutes. Page:7—18
35. Taxol-producing fungi: a new approach to industrial production of taxol. Page:1—6
36. Effect of homogeneity on cell growth and anthocyanin biosynthesis in suspension cultures of Vitis vinifera. Page:805—810
37. Production of gastrodin through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of Datura tatula L. Page:800—804
38. Preparation of new lipid-hydroxyapatite-DNA complex and gene transfection reseach in eukaryotic cell. Page:795—799
39. Rapid detection of Pseudomonas aeruginosa by the fluorescence quantitative PCR assay targeting 16S rDNA. Page:789—794
40. Transgenic mice produced by intracytoplasmic sperm injection. Page:784—788
41. Gene construction, expression and activities analysis of human leptin. Page:779—783
42. The effect of human IL-17F on growth of human hepatocarcinoma xenograft tumor in nude mice. Page:772—778
43. The synthesis and function analysis of omega-3 fatty acid desaturase gene from Caenorhabditis briggssae. Page:763—771
44. Activation of silent antibiotic synthesis in Streptomyces lividans by disruption of a negative regulator nsdA, a gene conserved in Streptomyces. Page:757—762
45. Gene cloning and expression analysis of a male sterility related gene BoDHAR from broccoli. Page:751—756
46. Produce of marker-free transgenic tobacco plants by FLP/frt recombination system. Page:744—750
47. Cloning, prokaryotic expression of chicken interferon-alpha gene and study on antiviral effect of recombinant chicken interferon-alpha. Page:737—743
48. Fusion expression of O type foot-and-mouth diseases virus VP1 gene and HSP70 gene and induction of immune responses in mice. Page:733—736
49. Cloning and analysis of phage Fab antibodies of mouse male specific antigen. Page:727—732
50. Generation high yield vaccine strain wholly derived from avian influenza viruses by reverse genetics. Page:720—726
51. The construction of recombinant adenovirus expressing bifunctional fusion protein sCAR-EGF and the detection of its activity. Page:713—719
52. Organization and transcription strategy of genome of Bombyx mori bidensovirus (China isolate) VD1. Page:707—712
53. Identification of mimotope peptides which bind to the SARS-CoV spike protein specific monoclonal antibody 2C5 with phage-displayed peptides library. Page:701—706
54. Progresses in the structure and function of Kazal-type proteinase inhibitors. Page:695—700
55. Studies on spermatogonial stem cells cultured in vitro of Wuzhishan Mini Porcine. Page:689—693
56. Screen of media for insect cells (HzAm1) growth and its adaptation to low serum culture. Page:686—688
57. Molecular cloning of an amidase gene from Nocardia sp. and its expressionin Escherichia coli. Page:682—685
58. Porcine follistatin cDNA cloning and expression in Escherichia coli. Page:677—681
59. Three-dimensional spheroid model for cultivating WB-F344 cells in simulated microgravity. Page:672—676
60. The effect of NAD kinase homologues on the beta-oxidation of unsaturated fatty acids with the double bond at an even position in Saccharomyces cerevisiae. Page:667—671
61. Hydroxylation of 16alpha, 17alpha-epoxy-4-pregenene-3, 20-dione by Absidia coerulea with pseudo-crystallo feed. Page:662—666
62. Optimization of SRAP & ISSR technology and its application in the identification of seeds of Brassica oleracea L. Page:657—661
63. Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides. Page:650—656
64. Isolation and identification of lipopeptides produced by Bacillus subtilis fmbJ. Page:644—649
65. Purification and biochemical character of a fibrinolytic protein from Eupolyphaga sinensis. Page:639—643
66. Purification and properties of isoflavone-glucosidase. Page:635—638
67. Production of polyclonal and monoclonal antibodies against citrus tristeza virus and their efficiency for the detection of the virus. Page:629—634
68. Effects of cu2+ on biosynthesis of camptothecin in cell cultures of Camptotheca acuminata. Page:624—628
69. Effects of exogenous phytohormones on hairy root growth and biosynthesis of anthraquinones in the hairy root culture of Polygonum multiflorum. Page:619—623
70. Effect of phosphate on growth and polysaccharide production by suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Page:613—618
71. Studies on relationship between the expression of hTNF-alpha gene and photosynthesis in Anabaena sp. IB02. Page:609—612
72. Simultaneous expression of modified hepatitis B surface antigen fusion polypeptides containing preS1, preS2 epitopes in Pichia pastoris. Page:604—608
73. Prokaryotic expression of fusion protein TAT-EDAG and study on its transduction activity. Page:598—603
74. Cloning and expression of pokeweed antiviral protein-II gene from the summer leaves of Phytolacca amercana. Page:592—597
75. Cloning of distinguishing DNA sequences of Gastrodia elata Blume and application of them in identifying gastrodia tuber. Page:587—591
76. A method using long primers for cloning the upstream sequence of delta-6 fatty acid desaturases gene of Thamnidium elegans by nested inverse PCR. Page:581—586
77. Improvement of baculovirus expression system and purification of IL-6 protein expressed in insect cells. Page:572—580
78. Overproduction of glucoamylase by recombinant Aspergillus niger harboring multiple copies of glaA. Page:567—571
79. Cloning and expression of Oreochromis aurea gonadotropin-releasing hormone cDNA in Escherichia coli and its immunogenicity for mice. Page:561—566
80. Construction and immunogenicity of recombinant adenovirus tandem expressing M and GP5 of porcine reproductive and respiratory syndrome virus. Page:555—560
81. A novel binary vector to get marker-free transgenic plant. Page:550—554
82. Expression of the gene coding for a thermostable alpha-amylase from Pyrococcus furious in Chiamydomonas reinhardtii chloroplast . Page:545—549
83. SCT-OGP fusion protein expression and its potential role as osteoporosis therapy. Page:539—544
84. Knockout of the hprK gene in B. subtilis CcpA mutant and its influence on riboflavin fermentation. Page:534—538
85. Improving phytase expression by increasing the gene copy number of appA-m in Pichia pastoris. Page:528—533
86. Fusion tags technology and their applications. Page:523—527
87. Induction of cucumber hairy roots and effect of cytokinin 6-BA on its growth and morphology. Page:514—520
88. Study on ethanol tolerance of Saccharomyces cerevisiae X330 under very high gravity medium. Page:508—513
89. A new purification methods of small DNAs--the purification methods with silica wool. Page:504—507
90. Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris. Page:499—503
91. Construction of recombinant yellow fever virus 17D containing 2A fragment as a vaccine vector. Page:492—498
92. Expression, purification and activity analysis of anti-human transferrin receptor scFv. Page:488—491
93. Fermentation process monitoring and fault detection based on dynamic MPCA. Page:483—487
94. Preparation and identification of soluble trimeric CD40-isoleucine zipper fusion protein. Page:477—482
95. Construction, expression and preliminary pharmacokinetic analysis of IL-1ra mutants. Page:472—476
96. Effect of sugars, gibberellic acid and abscisic acid on somatic embryogenesis in Tylophora indica (Burm. f.) Merrill. Page:465—471
97. Preparation of the cDNA microarray on the differential expressed cDNA of senescence-accelerated mouse's hippocampus. Page:457—464
98. Expression of human tumstatin in Pichia pastoris and its bioactivity. Page:451—456
99. Cloning of F gene of Newcastle disease virus HeB02 isolate and the study of its DNA vaccine. Page:445—450
100. Study on molecular identification and pre-harvest sprouting characteristic of the transgenic anti-trxs-gene wheat line 00T89. Page:438—444
101. Transgenic wheat (Triticum aestivum L.) with increased resistance to the storage pest obtained by Agrobacterium tumefaciens--mediated. Page:431—437
102. Construction of the eukaryotic expression vector with IL-2 gene and VP2 gene of PPV and research on immunogenicity. Page:425—430
103. Study of fusion protein and attachment glycoprotein of Nipah virus expressed in recombinant baculovirus. Page:418—424
104. Cloning and expression of human interleukin-26 in Escherichia coli. Page:413—417
105. Molecular cloning, tissue distribution and expression in engineered cells of human orphan receptor GPR81. Page:408—412
106. Analysis of the relationship between nm23-H1 gene and human chronic myeloblastic leukemia using siRNA. Page:403—407
107. The effect of oncolytic adenovirus on human umbilical vein endothelial cell. Page:397—402
108. Construction and immunological characterization of recombinant Marek's disease virus expressing IBDV VP2 fusion protein. Page:391—396
109. In vitro cytolysis of B-lymphoma cells mediated by an anti-CD3/anti-CD20 bispecific single-chain antibody. Page:384—390
110. Expression of sTNFR-IgGFc fusion gene in endothelial cell and its application in gene therapy for rheumatoid arthritis. Page:378—383
111. Construction and expression of various human prion proteins mutants with modified N-glycosylation sites in mammalian cells. Page:373—377
112. Construction and characterization of delta crp delta asd mutant host-vector balanced lethal system of Salmonella choleraesuis C500 strain. Page:366—372
113. Properties and advance of hepcidin. Page:361—365
114. Application of glycosyl fluorides in the study on glycosidases. Page:351—360
115. DC conductivity of DNA solution. Page:334—337
116. A review on genetic testing. Page:338—343
117. Inhibitory effect of new antimicrobial substance by Bacillus subtilis fmbJ on Newcastle disease virus and infectious Bursal disease virus in vitro. Page:328—333
118. Research of the feeding strategy in the fermentation of recombinant human fibreblast growth factor mutant. Page:322—327
119. Effects of temperature on the kinetics and level of energy charge and oxidation-reduction state in pyruvate biosynthesis. Page:316—321
120. The effect of induction temperature on aggregation of consensus interferon-alpha expressed by Pichia pastoris. Page:311—315
121. The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos. Page:306—310
122. Significant improved anthocyanins biosynthesis in suspension cultures of Vitis vinifera by process intensification. Page:299—305
123. A study on the discrimination of thermophilic and mesophilic proteins based on dipeptide composition. Page:293—298
124. Preparation of recombinant HIV-TATm-survivin (T34A) protein and its pro-apoptosis activity to cancer cells in vitro. Page:285—292
125. Development of a new hydrophobic interaction chromatography absorbent and its application to the purification of recombinant hepatitis B surface antigen. Page:278—284
126. Detection of intracellular reactive oxygen species by flow cytometry in Pichia pastoris fermentation. Page:273—277
127. Effect of feeding pre-L-methionine on high-cell-density fermentation for S-adenosyl-L-methionine production. Page:268—272
128. Study on the asexual sporulation of Aspergillus niger under blue light induction and analysis of its subtractive library. Page:263—267
129. Effect of straw apoplast protein on cellulase activity. Page:257—262
130. Recombinant expression and secretion of mpd gene using the promoters and signal peptide-encoding sequences of ytkA and ywoF gene from Bacillus subtilis. Page:249—256
131. Construction of human growth hormone lentiviral vector and its expression in murine skeletal myoblasts. Page:243—248
132. Molecular cloning and expression of extremely thermostable and acid-stable amylase gene in Pichia pastoris. Page:237—242
133. Expression of AIV subtype H5HA, H7HA and H9HA hemagglutinin gene in Pichia pastoris. Page:231—236
134. Expression of human LNalpha4LG4-5 in Pichia yeast. Page:226—230
135. Construction of VEGF recombinant plasmid pcDNA/V and its expression in model rats with acute myocardial ischemia. Page:220—225
136. Comparative study on in vitro stationary culture system or superfusion culture system of cow mammary tissue. Page:1040—1046
137. Expression of porcine beta-defensin 1 gene in Pichia pastoris. Page:1036—1039
138. Design and implementation of DB sequence optimization software. Page:1032—1035
139. Application of boosting-based decision tree ensemble classifiers for discrimination of thermophilic and mesophilic proteins. Page:1026—1031
140. The influences of lactose as an inducer on expression of plant des-pGlu1-brazzein in Escherichia coli. Page:1021—1025
141. Study on application of rep-PCR fingerprint in rapid identification of beer-spoilager. Page:1013—1020
142. Methane monooxygenases hydroxylase from a type II methanotroph: purification and physical-chemical properties. Page:1007—1012
143. Effects of baicalein on the proliferation and differentiation of pig preadipocyte. Page:1002—1006
144. Expression and characterization of VPAC2 in CHO cells. Page:996—1001
145. Establishment and application of human papillomavirus type 16 pseudovirions neutralization assay. Page:990—995
146. Impact of subculture cycles and inoculum sizes on suspension cultures of Vitis vinifera. Page:984—989
147. Selecting the RNAi efficiency target sites of lZP3 gene by mouse in vivo. Page:979—983
148. Expression of single chain fragment variable P1D3 antibody against shrimp white spot syndrome virus in Pichia pastoris. Page:973—978
149. Expression, characterization and biological activity analysis of recombinant human bone morphogenetic protein 2 in CHO cells. Page:968—972
150. Expression and purification of recombinant human interleukin 4 in Escherichia coli. Page:962—967
151. Expression of recombinant human cytomegalovirus fusion proteins pp150/MDBP fragments and its application. Page:956—961
152. Cloning and expression of the genes encoding glycerol dehydratase reactivase and identification of its biological activity. Page:950—955
153. Isolation, sequencing analysis and characterization of the promoter of banana lectin gene. Page:945—949
154. Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis. Page:940—944
155. Construction and characterization of a recombinant fowlpox virus co-expressing F, HN genes of Newcastle disease virus and gB gene of infectious laryngnotracheitis virus. Page:931—939
156. The study of the growth-suppression and mechanisms of hepatocelluar carcinoma tumor in nude mice. Page:925—930
157. New option for gene-targeting vector construction Red/ET recombination. Page:919—924
158. Construction and expression of HSV-2gD-Hsp70 fusion protein gene. Page:914—918
159. Expression and secretion of human bone morphogenetic protein-7 in Pichia pastoris. Page:907—913
160. Cloning and analysis of geldanamycin partial biosynthetic gene cluster of Streptomyces hygroscopicus 17997. Page:902—906
161. In silico cloning of Efp-0, a novel earthworm fibrinolytic enzyme gene and verification of its coding region by RT-PCR. Page:897—901
162. Advances in the RNA-directed DNA methylation in plants. Page:891—896
163. Specific promoters used in plant gene engineering. Page:882—890
164. Advances in heavy metal ions immunoassay. Page:877—881
165. Research progress in SELDI-TOF MS and its clinical applications. Page:871—876
166. The advancement of AFLP technology. Page:861—865
167. Application of optical proteinchip in detecting phage M13KO7. Page:856—860
168. Effects of resveratrol on pig primary preadipocytes proliferation, differentiation and transcription expression of Sirt1 gene. Page:850—855
169. Study on vitrification of porcine embryos by open pulled straw method. Page:845—849
170. Process performance of simultaneous anaerobic sulfide and nitrate removal. Page:840—844
171. Isolation and characterization of gamma-TMT gene promoter from Arabidopsis thaliana. Page:835—839
172. The preparation of an elicitor from a fungal endophyte to enhance artemisinin production in hairy root cultures of Artemisia annua L. Page:829—834
173. Model study on CO2 removal by photobioreactor. Page:821—828
174. Continuous ethanol fermentation coupled with recycling of yeast flocs. Page:816—820
175. Refolding of the fusion protein of recombinant enterokinase light chain rEKL. Page:811—815