WPRIM Management System> DCMS> Chinese Journal of Biotechnology> 2004> 20> 1

Volume: 20 Issue: 1

1. Construction of recombinant fowlpox virus expressing chicken IL-2 and assay of biologic activity of the product in vitro. Page:136—139
2. Expressing of N gene encoding nucleocapsid protein of vesicular stomatitis virus and elementary application in ELISA. Page:130—135
3. Fermentation, purification and identification of recombinant RGD-Hirudin. Page:126—129
4. Transgenic maize plants with low copy number of foreign genes were produced with maize Ubi-1 promoter. Page:120—125
5. Addition of TCA cycle intermediates enhances pyruvate production. Page:115—119
6. Oriented immobilization of human IgG by protein A on imaging ellipsometry biosensor. Page:111—114
7. Participation of ions and solutes on the thermostability of alpha-amylase. Page:104—110
8. Cloning of human beta-microglobulin gene and its high expression in Escherichia coli. Page:99—103
9. Interface domain of hepatitis E virus capsid protein homodimer. Page:90—98
10. Physical and chemical characters of recombinant human nucleoside diphosphate kinase A. Page:85—89
11. Overexpression of Escherchia coli phytase with high specific activity. Page:78—84
12. Mammalian gene-transfer and expression efficiencies of baculovirus bacV-CMV-EGFPA. Page:73—77
13. Construction of two robust CHO cell lines resistant to apoptosis and adapted to protein-free medium by over-expression of Igf-1/bcl-2 or bcl-2/cyclin E genes. Page:66—72
14. Preparation of alpha-toxin's protective antigen of clostridium perfringens type A and research for its primary immunological protective function. Page:63—65
15. Identification of an auxin response factor-like protein cDNA from mango cotyledon section. Page:59—62
16. Construction of cDNA library from NPC tissue and screening of antigenic genes. Page:54—58
17. Expression and purification of three fusion proteins containing a single B-cell epitope (beta5, beta9 or beta8) of human chorionic gonadotropin beta subunit. Page:49—53
18. Construction of combined site-directed random mutation libraries of recombinant human lymphotoxin. Page:43—48
19. Identification of genes that are specifically/preferentially expressed in developing cotton fibers by mRNA fluorescence differential display (FDD). Page:39—42
20. Expression of delta6-fatty acid desaturase gene from Mortierella alpina in Pichia pastoris. Page:34—38
21. Cloning and expression of hTRF1 in Escherichia coli and preparation of polyclonal antibody. Page:30—33
22. Expression of TMP Fc in Pichia pastoris and identification of its biological activity. Page:25—29
23. Expression of goat beta-casein gene targeting vector in mammary gland cell. Page:21—24
24. Knockout of the ptsG gene in Escherichia coli and cultural characterization of the mutants. Page:16—20
25. Using green fluorescent protein as a reporter to monitor elimination of selectable marker genes from transgenic plants. Page:10—15
26. The potential role of nuclear matrix attachment regions (MARs) in regulation of gene expression. Page:6—9
27. Progression and direction of humanized antibody research. Page:1—5