WPRIM Management System> DCMS> Chinese Journal of Biotechnology> 2002> 18

Volume: 18

1. Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris. Page:348—351
2. In vitro culture of umbilical cord blood MNC and CD34+ selected cells. Page:343—347
3. Site-directed mutation of PoIFN-alpha and its expression in Escherichia coli. Page:339—342
4. A novel resolution vector with Bacillus thuringiensis plasmid replicon ori44. Page:335—338
5. Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli. Page:331—334
6. Construction and in vitro activity of specific dual-ribozyme against alpha 1 (I) and (III) procollagen genes. Page:327—330
7. Callus induction and regeneration from mature seeds of indica rice minghui 63 and anti-fungal assay of transgenic rice plants. Page:323—326
8. Study on optimization of expression, purification, properties and biological function of recombinant human sBLyS. Page:318—322
9. Screening for resistance gene candidate from a genomic TAC library of Triticum aestivum-Haynaldia villosa translocation line 6VS/6AL by pooled PCR. Page:313—317
10. Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker. Page:308—312
11. Overexpression and detection of the mutated glucose isomerase GIG138P and GIG138P-G247D in Streptomyces lividans. Page:304—307
12. The expression of Aeromonas hydrophila ompTS gene and the immunogenicity of recombinant OmpTS. Page:300—303
13. Production of SAM by recombinant Pichia pastoris. Page:295—299
14. Refolding and purification of the huGM-CSF(9-127)-IL-6(29-184) fusion protein. Page:291—294
15. A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue. Page:286—290
16. The development of peptide nucleic acid in gene regulation. Page:282—285
17. Gene and gene engineering of carotenoid biosynthesis. Page:276—281
18. Progresses in mouse chromosome engineering. Page:272—275
19. Development of the genetic transformation system in extremely halophilic archaea. Page:267—271
20. Escherichia coli disulfide-forming related proteins: structures, functions and their application in gene engineering for expressing heterologous proteins in Escherichia coli. Page:261—266
21. The electron microscopic in situ hybridization and its application. Page:249—252
22. Protein microarrays and their medical applications. Page:246—248
23. Lipase-catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester in organic solvent. Page:242—245
24. Study of alginate/chitosan microcapsules for immobilization of Escherichia coli DH5 alpha. Page:239—241
25. Study on the kinetics of immobilized cells of Brevibacterium ammoniagenes MA-2 and Brevibacterium flavum MA-3. Page:235—238
26. Protective effect induced by vaccination with a partial cDNA expression library of Schistosoma japonicum in mice. Page:231—234
27. Study on production of acrylamide by microbial method (II)--enzyme catalytic kinetics and de-active dynamics of nitrile hydratase. Page:225—230
28. Study of immobilization of Aspergillus oryzae mycelium aided by artificial neural network. Page:221—224
29. Construction of recombinant strain expressing enterotoxigenic Escherichia coli K88ac-ST1-LTB fusion protein. Page:216—220
30. Cloning and expression of spinach glycolate oxidase in Escherichia coli. Page:212—215
31. Study on the expression of E2 gene of classical swine fever virus in Pichia pastoris and the immunological activity of its expression product. Page:208—211
32. Induced-effects by additional taxol in suspension cultures of Taxus cuspidata cells. Page:204—207
33. Construction of Saccharopolyspora erythraea M synthesizing a novel ketolide 3-deoxy-3-oxo-erythronolide B. Page:198—203
34. Expression of adenovirus-mediated pGH cDNA with first intron in CHO cells. Page:193—197
35. Expression and characterization of envelope protein 2 gene of hepatitis G virus in Pichia pastoris. Page:187—192
36. Cloning of Xanthomonas campestris pv. campestris pathogenicity-related gene sequences by TAIL-PCR. Page:182—186
37. Cloning, expression and bio-activity assay of chimeric fusion protein sTNFRII-IgG Fc. Page:178—181
38. Expression and purification of recombinant huwentoxin-I in Pichia pastoris. Page:172—177
39. Construction of the mutants of rice nonspecific lipid transfer protein and expression comparison in two kinds of thioredoxin fusion expression vectors. Page:167—171
40. Tagging and mapping of QTLs controlling lint yield and yield components in upland cotton (Gossypium hirsutum L.) using SSR and RAPD markers. Page:162—166
41. Cloning, sequencing and high expression in Escherichia coli of D-hydantoinase gene from Burkholderia pickettii. Page:149—154
42. Transgenic animals bioreactors. Page:144—148
43. The mechanism and application of posttranscriptional gene silencing. Page:140—143
44. The progress of expressing vector for mammary gland bioreactor. Page:136—139
45. Current state of establishing and maintaining human embryonic stem cell lines and key problems in these studies. Page:131—135
46. Visualization of the interaction between IL-6 and IL-6R by imaging ellipsometry. Page:99—101
47. Studies on lipase-catalyzed synthesis of ethylglucoside lactate in non-aqueous phase. Page:94—98
48. Continuous biosynthesis of epoxypropane in a methanotrophic attached-films reactor. Page:89—93
49. Enzymatic synthesis of monoglycerides in microaqueous media by using lipase from Pseudomonus fluorescens. Page:84—88
50. Cloned goats produced from the somatic cells of an adult transgenic goat. Page:79—83
51. Studies on the optimal expression condition, purification and its characterization of ScFv-2F3. Page:74—78
52. Hairy-root culture of Polygonium multiflorum Thunb. and the production of its active constituents-anthraquinones. Page:69—73
53. Cloning and expression of a thermostable beta-glycosidase gene from Thermus nonproteolyticus HG102. Page:63—68
54. Effect of trypsin on the rat keratinocyte separation and subculture. Page:59—62
55. Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase. Page:55—58
56. Extraction of the salt-tolerant cDNA in mangrove Avicennia marina by mRNA differential display. Page:51—54
57. Avirulence genes of plant pathogens. Page:5—9
58. Nucleotide sequence and protein sequence analysis of GL-7-ACA acylase from Pseudomonas sp. 130. Page:45—50
59. Expression of the human soluble low density lipoprotein receptor in methylotropic yeast. Page:40—44
60. Cloning and expression of extracellular domain of prostate specific membrane antigen in Escherichia coli and preparation of polyclonal antibody. Page:35—39
61. Expression of human interleukin-11 in cell culture and larvae of silkworm. Page:30—34
62. The construction of the hammerhead ribozyme genes targeting against apple scar skid viroid and its activity detection in vitro. Page:25—29
63. Couple production of human calcitonin and rat peptidylglycine alpha-amidation monooxygenase in insect cells. Page:20—24
64. Na+/H+ antiporter and plant salt tolerance. Page:16—19
65. The thinking about modern biological technology. Page:121—123
66. Strategies of functional analysis of new genes. Page:117—120
67. Cloning of 5', 3' flanking sequence of ovine BLG and regulating the expression of GFP in mammary gland cell line. Page:112—116
68. Attachment and growth of cultured fibroblast cells on chitosan/PHEA-blended hydrogels. Page:109—111
69. Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli. Page:106—108
70. Genome analysis of transgenic homozygous line "Minghui 63-Xa21". Page:102—105
71. Bioactive compounds from marine sponges and cell culture of marine sponges. Page:10—15
72. Molecular biological progress of hepatocyte growth factor (HGF). Page:1—4
73. Cloning and expression of 21.7 kD protein gene of Schistosoma japonicum (Chinese strain). Page:698—702
74. Progress in purification of human serum albumin. Page:761—766
75. The study on the stable inheritance and expression of foreign gene in transgenic mice. Page:758—760
76. Effects of SCF, LIF and bFGF on mouse spermatogonial stem cells proliferation in vitro. Page:754—757
77. The construction of cDNA expression library from the tentacles of Sagartia rosea. Page:749—753
78. Structure characteristics of ORF2a gene of potato leafroll virus Chinese isolate. Page:744—748
79. Discussion of the methods for establishing embryonic stem cell lines from 129/ter. C57BL/6J mice with high efficiency. Page:740—743
80. Synthesis of partial glycerol caprates by using lipase in nonaqueous media. Page:735—739
81. Study on fermentation condition of alkaline protease gene engineering strain and the purification and characterization of recombinant enzyme. Page:729—734
82. Utilization of sugar cane bagasse hydrolysates for xylitol production by yeast. Page:724—728
83. High density fed-batch culture of Escherichia coli DH5 alpha/pDH-B2m with DO feed-back control of nutrient feeding. Page:718—723
84. The progressive study on gene therapy for hyperphenylalaninemia rats. Page:713—717
85. Biocompatibility of SA/CS-CaCl2/PMCG Microcapsule in cell culture. Page:709—712
86. Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins. Page:703—708
87. The renaturation and purification of RGD-staphylokinase by gel filtration. Page:693—697
88. Cloning and expression product of vip3A gene from Bacillus thuringiensis and analysis of inseceicidal activity. Page:687—692
89. Expression of porcine interferon-gamma gene in Pichia pastoris and its effect of inhibiting porcine reproductive and respiratory syndrome virus. Page:683—686
90. Minimal functional domain of cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuAc) synthetase from Escherichia coli. Page:676—682
91. Comparative analysis of diuretic activities of human ANP gene injected intramuscularly and intravenously in adriamycin-induced nephrotic rats. Page:671—675
92. Construction of recipient strain of expression-secretion system in filamentous fungi. Page:667—670
93. Cloning of the replication origin (oriC) of Streptoverticillum caespitosus and transformation of Streptomyces lividans ZX7. Page:662—666
94. Mapping of BRCT1 domain of BRCA1 with chromatin unfolding activity. Page:656—661
95. Progress in biotechnological production of pyruvic acid. Page:651—655
96. The PTD domain of Tat protein enhance GFP protein delivering into myeloma cell SP2/0. Page:644—647
97. Amplification, cloning and sequence analysis of spider dragline silk cDNA. Page:641—643
98. Induction of petal-like structures from petals of Crocus sativus L. and the differentiation of style-stigma-like structures in vitro. Page:638—640
99. Influence of pH control on the production of curdlan by Alcaligenes faecalis strain. Page:634—637
100. The effect of antioxidants on the in vitro life-span of keratinocyte. Page:630—633
101. Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes. Page:626—629
102. Analysis on metabolic flux shift during guanosine fermentation. Page:622—625
103. Micropatterning of biotin-avidin layers and cell location. Page:619—621
104. Biosynthesis and accumulation of poly(3-hydroxybutyrate) in Vibrio natriegens. Page:614—618
105. Preparation of bovine lipid-free hemoglobin. Page:609—613
106. Cloning of the major antigen region of E2 gene of hog cholera virus and expression in Escherichia coli. Page:605—608
107. Induction of immune responses in mice by hepatitis B virus large envelope DNA vaccine delivered by attenuated Salmonella typhimurium. Page:601—604
108. Cloning of staphylococcal enterotoxin B gene and its highly expression in Escherichia coli. Page:597—600
109. Cloning and expression of kringle 1-3 gene of human plasminogen and the purification and bioactivity of its expressed product. Page:593—596
110. Design and preparation of the multimeric self-cleavable hammerhead ribozyme targeting apple scar skid viroid and its activity detection in vitro. Page:588—592
111. Characterization of inductive synthesis of levoglucosan kinase by a combined strategy of enzymological and fast atom bombardment mass spectrometric analysis. Page:583—587
112. The analysis of Bacillus thuringiensis vegetative insecticical protein gene cloning and expression. Page:578—582
113. BjCHI1 from Brassica juncea displays both chitinase and agglutination activity. Page:572—577
114. Cloning and sequencing of lactase gene from Aspergillus candidus. Page:566—571
115. Purification and characterization of antifungal proteins in triticale seed. Page:561—565
116. Screening of TNF-alpha antagonist peptides from a random peptide library displayed with Escherichia coli flagellar. Page:556—560
117. Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator. Page:551—555
118. The expression of humanized Fab fragment of the anti-HBsAg antibody in methylotropic yeast Pichia pastoris. Page:546—550
119. Fusion expression of human thymosin alpha 1 in Escherichia coli. Page:541—545
120. High expression and identification of DNA mismatch repair gene mutS in Escherichia coli. Page:536—540
121. Progress in the study of the structure and function of Cre recombinase. Page:531—535
122. The methods of identifying differences in mRNA expression. Page:521—524
123. Food-grade gene expression systems for lactic acid bacteria. Page:516—520
124. Differences in gene expression between Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase. Page:512—515
125. The functional expression of humanized ScFv-urokinase fusion protein in Escherichia coli. Page:509—511
126. Enhancing hGH expression level in insect cells by shortening the 5'-UTR of hGH cDNA. Page:505—508
127. Preparation and analysis of oligonucleotide microarray for expression detection of mouse cytokine-associated gene. Page:501—504
128. Expression of cre gene in Escherichia coli and bioassay its expression product. Page:497—500
129. Performance of internal-loop air-lift nitrifying bioreactor. Page:492—496
130. Hydrogen photoproduction from acetate by Rhodopseudomonas palustris. Page:486—491
131. Analysis of salt resistance on the poplar transferred with salt tolerance gene. Page:481—485
132. The changes of rare codons increase expression level of SEA. Page:477—480
133. Expression of polyprotein of infectious bursal disease virus in Bombyx mori. Page:472—476
134. Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal. Page:468—471
135. The study of aggregate of the ORF2 peptide of hepatitis E virus expressed in Escherichia coli. Page:463—467
136. Studies on production, purification and partial characteristics of the extracellular laccase from Armilliria mellea. Page:457—462
137. Analysis of the proteomic changes of the serum of the Smad3 targeted deficient mice. Page:452—456
138. Detection of P53 and K-ras gene mutations in lung cancer with oligonucleotide chip. Page:447—451
139. Anther culture generated stem borer-resistance DH lines of Minghui 81(Oryza sativa L. subsp. indica) expressing modified cry1Ac gene. Page:442—446
140. The construction of shuttle vectors of Brevibacillus brevis-Escherichia coli. Page:438—441
141. Establishment of gene replacement/disruption system through homologous recombination in Amycolatopsis mediterranei U32. Page:431—437
142. Expression of TPO mimetic peptide chimeric proteins with human IgG1 Fc fragments and their biological characters. Page:424—430
143. Construction of a temperature inducible shuttle expression vector and its application in Streptomyces. Page:420—423
144. Modification of Chinese hamster ovary cells. Page:415—419
145. Agroinfiltration, a useful technique in plant molecular biology research. Page:411—414
146. Advances in the application research of bacterial ice nucleation active (ina) genes. Page:407—410
147. Current researches on molecular enzyme engineering. Page:401—406
148. Studies on improving crop nutritional quality through gene engineering. Page:381—386
149. Technological advances of serial analysis of gene expression. Page:377—380
150. Optimization of cultivation conditions in se-enriched Spirulina platensis. Page:373—376
151. Effect of microbial elicitors on sclerotia biomass and carotenoid yield in surface cultures of Penicillium sp.PT95. Page:369—372
152. Cloning and expression of canine interferon-gamma gene in mouse SP2/0 cell line. Page:365—368
153. Continuous perfusion culture hybridoma cells for production of monoclonal antibody. Page:360—364
154. Single-chain urokinase-type plasminogen activator (scu-PA) purification by immuno-affinity chromatography. Page:356—359
155. Cloning and expression of the cecropin B-thanatin hybrid antimicrobial peptide in Escherichia coli. Page:352—355