WPRIM Management System> DCMS> Chinese Journal of Biotechnology> 2002> 18> 3

Volume: 18 Issue: 3

1. Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris. Page:348—351
2. In vitro culture of umbilical cord blood MNC and CD34+ selected cells. Page:343—347
3. Site-directed mutation of PoIFN-alpha and its expression in Escherichia coli. Page:339—342
4. A novel resolution vector with Bacillus thuringiensis plasmid replicon ori44. Page:335—338
5. Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli. Page:331—334
6. Construction and in vitro activity of specific dual-ribozyme against alpha 1 (I) and (III) procollagen genes. Page:327—330
7. Callus induction and regeneration from mature seeds of indica rice minghui 63 and anti-fungal assay of transgenic rice plants. Page:323—326
8. Study on optimization of expression, purification, properties and biological function of recombinant human sBLyS. Page:318—322
9. Screening for resistance gene candidate from a genomic TAC library of Triticum aestivum-Haynaldia villosa translocation line 6VS/6AL by pooled PCR. Page:313—317
10. Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker. Page:308—312
11. Overexpression and detection of the mutated glucose isomerase GIG138P and GIG138P-G247D in Streptomyces lividans. Page:304—307
12. The expression of Aeromonas hydrophila ompTS gene and the immunogenicity of recombinant OmpTS. Page:300—303
13. Production of SAM by recombinant Pichia pastoris. Page:295—299
14. Refolding and purification of the huGM-CSF(9-127)-IL-6(29-184) fusion protein. Page:291—294
15. A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue. Page:286—290
16. The development of peptide nucleic acid in gene regulation. Page:282—285
17. Gene and gene engineering of carotenoid biosynthesis. Page:276—281
18. Progresses in mouse chromosome engineering. Page:272—275
19. Development of the genetic transformation system in extremely halophilic archaea. Page:267—271
20. Escherichia coli disulfide-forming related proteins: structures, functions and their application in gene engineering for expressing heterologous proteins in Escherichia coli. Page:261—266
21. Studies on improving crop nutritional quality through gene engineering. Page:381—386
22. Technological advances of serial analysis of gene expression. Page:377—380
23. Optimization of cultivation conditions in se-enriched Spirulina platensis. Page:373—376
24. Effect of microbial elicitors on sclerotia biomass and carotenoid yield in surface cultures of Penicillium sp.PT95. Page:369—372
25. Cloning and expression of canine interferon-gamma gene in mouse SP2/0 cell line. Page:365—368
26. Continuous perfusion culture hybridoma cells for production of monoclonal antibody. Page:360—364
27. Single-chain urokinase-type plasminogen activator (scu-PA) purification by immuno-affinity chromatography. Page:356—359
28. Cloning and expression of the cecropin B-thanatin hybrid antimicrobial peptide in Escherichia coli. Page:352—355