Correlation between Gene Expression of MDR1, Cyclin B, MAD2 and BAX in Childhood Acute Lymphocytic Leukemia.
- Author:
Won Duck KIM
1
;
Taeg Kyu KWON
;
Kwang Hae CHOI
;
Jeong Ok HAH
Author Information
1. Department of Pediatrics, Yeungnam University Medical Center, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
MDR1;
Cyclin B;
MAD2;
BAX;
ALL
- MeSH:
Actins;
Apoptosis;
Bone Marrow;
Cell Cycle;
Cell Cycle Checkpoints;
Child;
Cyclin B*;
Cyclins*;
Drug Therapy;
Gene Expression*;
Humans;
Precursor Cell Lymphoblastic Leukemia-Lymphoma*;
Recurrence;
RNA, Messenger;
Sample Size;
Treatment Failure
- From:Korean Journal of Pediatric Hematology-Oncology
2000;7(1):57-63
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Although outcome of the children with ALL has been improved remarkably with the multidrug chemotherapy and supportive therapy, relapse is still important cause of treatment failure. One of the mechanism related to the relapse has been reported to be multidurg resistance (MDR). To investigate the relation between the expression of MDR related MDR1 gene and cell cycle and apoptosis related genes in children with ALL, this study was conducted. METHODS: The samples were collected from 9 children with ALL (5: at presentation, 4: at relapse) diagnosed at the pediatric department of Yeungnam University Hospital. From the mononuclear cells isolated from the peripheral blood or bone marrow, mRNA was extracted and analysed by RT-PCR. Using actin as a control, relative levels of mRNA of MDR1 gene, cell cycle control protein cyclin B and MAD2 and apoptosis related BAX gene were analysed. RESULTS: The expression of MDR1 gene at the presentation and the relapse were variable and showed high correlation (Pearson correlation: 0.826) with the expression of BAX gene but low correlation with the expression of cyclin B and MAD2 gene. CONCLUSION: These results suggest that mechanism involved in relapse of ALL include mechanisms other than MDR1 gene. High correlation between the expression of MDR1 gene and BAX gene suggests that high level of BAX expression increases probability of relapse but small sample size of this study precludes definite conclusion and further study is needed.
