Meclofenamate Suppresses MUC5AC Mucin Gene Expression by Regulating the NF-kB Signaling Pathway in Human Pulmonary Mucoepidermoid NCI-H292 Cells
10.4062/biomolther.2023.035
- Author:
Jiho RYU
1
;
Kyung-il KIM
;
Rajib HOSSAIN
;
Misoon LEE
;
Jin Tae HONG
;
Hyun Jae LEE
;
Choong Jae LEE
Author Information
1. Department of Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea
- Publication Type:Original Article
- From:Biomolecules & Therapeutics
2023;31(3):306-311
- CountryRepublic of Korea
- Language:English
-
Abstract:
The current study aimed to reveal the potential effect of meclofenamate, a nonsteroidal anti-inflammatory drug, on the gene expression of airway MUC5AC mucin. Human pulmonary mucoepidermoid NCI-H292 cells were pretreated with meclofenamate for 30 min and stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. Thereafter, the effect of meclofenamate on the PMAinduced nuclear factor kappa B (NF-kB) signaling pathway was assessed. Meclofenamate inhibited glycoprotein production and mRNA expression of MUC5AC mucins induced by PMA by inhibiting the degradation of inhibitory kappa Bα (IkBα) and NF-kB p65 nuclear translocation. These results suggest meclofenamate suppresses mucin gene expression by regulating NF-kB signaling pathway in human pulmonary epithelial cells.
