Establishment of HPLC fingerprint and content determination of 8 components in Jianpi huayu decoction
- VernacularTitle:健脾化瘀方的HPLC指纹图谱建立及8种成分含量测定
- Author:
Xiaokai CHEN
1
;
Yong LI
2
;
Wei TAN
3
,
4
;
Rongjian YU
3
,
4
;
Jianjiang HUANG
5
;
Zhixin LIU
2
;
Junze LIN
3
,
4
;
Changjun WANG
3
Author Information
1. Dept. of Pharmacy，Guangdong Provincial People’s Hospital （Guangdong Academy of Medical Sciences），Southern Medical University，Guangzhou 510080，China
2. Guangdong Provincial Key Laboratory of Advanced Drug Delivery and Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System，Guangdong Pharmaceutical University，Guangzhou 510006，China
3. Dept. of Traditional Chinese Medicine，Guangdong Provincial People’s Hospital （Guangdong Academy of Medical Sciences），Southern Medical University，Guangzhou 510080，China
4. Dept. of Traditional Chinese Medicine，Guangdong Institute of Geriatrics，Guangzhou 510080，China
5. Dept. of Pathology，Guangdong Provincial People’s Hospital （Guangdong Academy of Medical Sciences），Southern Medical University，Guangzhou 510080，China
- Publication Type:Journal Article
- Keywords:
Jianpi huayu decoction;
fingerprint;
content determination;
HPLC;
quality standard
- From:
China Pharmacy
2023;34(23):2874-2879
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the HPLC fingerprint of Jianpi huayu decoction， and to determine the contents of 8 components. METHODS Thermo Hypersil Gold C18 column was used with mobile phase consisted of methanol-0.05% phosphoric acid aqueous solution （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃， the injection volume was 5 μL. The detection wavelength of matrine was 211 nm， and the other components’ detection wavelength was 283 nm. The similarity evaluation of HPLC fingerprints for 10 batches of Jianpi huayu decoction was performed by using the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition）. The contents of chlorogenic acid， vanillic acid， p-coumaric acid， ferulic acid， hesperidin， quercetin， bergapten and matrine in the samples were determined by HPLC. RESULTS HPLC fingerprint of Jianpi huayu decoction was established. A total of 27 common peaks were identified， and 8 components were identified. The similarity between 10 batches of samples and the control map ranged from 0.942-0.999. RSDs of precision， repeatability and stability tests were less than 3% （n＝6）. The average recoveries of chlorogenic acid， vanillic acid， p-coumaric acid， ferulic acid， hesperidin， quercetin， bergapten and matrine were 99.48%， 101.32%， 101.18%， 100.79%， 101.12%， 99.19%， 99.81% and 102.46%， respectively； RSDs were 1.34%， 0.93%， 1.90%， 1.84%， 0.54%， 1.53%， 1.33% and 1.01%， respectively （n＝6）. The contents were 0.021-0.061， 0.025-0.034， 0.116-0.295， 0.006- 0.062， 0.014-0.053， 0.017-0.026， 0.014-0.027 and 14.05-24.11 mg/g， respectively. CONCLUSIONS The established fingerprint and content determination method can provide a reference for the quality control and subsequent preparation development for Jianpi huayu decoction.
