HPLC fingerprint，content determination and transferring patterns of index components of Xintongshu spray
- VernacularTitle:心痛舒喷雾剂HPLC指纹图谱、含量测定及指标成分传递规律研究
- Author:
Wanhui LIANG
1
;
Shuyue TAO
1
;
Chijing ZUO
1
;
Jie WANG
1
;
Yan LIU
1
;
Weidong YU
1
;
Can PENG
1
,
2
,
3
,
4
,
5
;
Qian WANG
1
Author Information
1. School of Pharmacy，Anhui University of Chinese Medicine，Hefei 230012，China
2. MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials，Hefei 230012，China
3. Rural Revitalization Collaborative Technical Service Center of Anhui Province，Hefei 230012，China
4. Generic Technology Research Center for Anhui TCM Industry，Hefei 230012，China
5. Anhui Provincial Key Laboratory of Pharmaceutical Preparation Technology and Application，Hefei 230012，China
- Publication Type:Journal Article
- Keywords:
Xintongshu spray;
HPLC;
fingerprint;
content determination;
transferring patterns of components
- From:
China Pharmacy
2023;34(23):2861-2867
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the HPLC fingerprint of Xintongshu spray， determine the contents of identified components， and investigate the transferring patterns of the index components of decoction pieces， intermediates and spray， so as to provide scientific reference for technology management and quality control of Xintongshu spray. METHODS HPLC fingerprints of 13 batches of Xintongshu spray were established by the Similarity Evaluation System for Chromatographic Fingerprints of TCM （2012 edition）， and common peaks were identified； the contents of identified components were determined by HPLC. The paeonol in Moutan Cortex and ferulic acid in Chuanxiong Rhizoma were used as index components to investigate the transferring patterns of them in decoction pieces， intermediates and spray. RESULTS There were a total of 33 common peaks in the fingerprints of 13 batches of Xintongshu spray， and the similarities were more than 0.994. Eight components were identified， i.e. gallic acid （peak 5）， oxypaeoniflorin （peak 9）， chlorogenic acid（peak 10）， caffeic acid （peak 14）， paeoniflorin （peak 17）， ferulic acid （peak 21）， senkyunolide Ⅰ （peak 27） and paeonol （peak 31）. The contents of 8 components ranged from 0.590 3- 0.719 7， 0.565 7-0.851 3， 0.279 4-0.368 1， 0.080 6-0.106 1， 1.922 5-3.033 5， 0.151 3-0.191 6， 0.250 6-0.336 0， 3.056 7-4.161 0 mg/mL， respectively. The average transfer rates of paeonol and ferulic acid from decoction pieces to sprays were 63.76% and 38.06%， respectively. It was also found that the process in which the loss of paeonol was more than 30% was the extraction by percolation and negative pressure concentration of Moutan Cortex. The process in which the loss of ferulic acid was more than 50% was the steam distillation extraction process of Chuanxiong Rhizoma. CONCLUSIONS The established HPLC fingerprint and content determination method of Xintongshu spray are reproducible and specific. The key processes that cause a decrease in the average transfer rates of the index components are the extraction by percolation and negative pressure concentration of Moutan Cortex and steam distillation extraction of Chuanxiong Rhizoma.
