Preparation of mesoporous silica nanoparticles with different sizes and study on the correlation between size and toxicity
10.16438/j.0513-4870.2023-0011
- VernacularTitle:不同粒径介孔二氧化硅纳米颗粒的制备及其与毒性的相关性研究
- Author:
Xiao-wei XIE
;
Meng-ying CHENG
;
Wei-xiang FANG
;
Xue LIN
;
Wen-ting GU
;
Kai-ling YU
;
Ting-xian YE
;
Wei-yi CHENG
;
Li HE
;
Hang-sheng ZHENG
;
Ying-hui WEI
;
Ji-gang PIAO
;
Fan-zhu LI
- Publication Type:Research Article
- Keywords:
mesoporous silica nanoparticle;
particle size;
size-dependent toxicity;
oxidative stress;
nanotoxicity
- From:
Acta Pharmaceutica Sinica
2023;57(8):2512-2521
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the crucial role of particle size in the biological effects of nanoparticles, a series of mesoporous silica nanoparticles (MSNs) were prepared with particle size gradients (50, 100, 150, 200 nm) with the traditional Stober method and adjusting the type and ratio of the silica source. The correlation between toxicity and size-caused biological effects were then further examined both in vitro and in vivo. The results indicated that the prepared MSNs had a uniform size, good dispersal, and ordered mesoporous structure. Hemolytic toxicity was found to be independent of particle size. At the cellular level, MSNs with smaller particle sizes were more readily internalized by cells, which initiated to more intense oxidative stress, therefor inducing higher cytotoxicity, and apoptosis rate. In vivo studies demonstrated that MSNs primarily accumulated in the liver and kidneys of mice. Pharmacokinetic analysis revealed that larger MSNs were eliminated more efficiently by the urinary system than smaller MSNs. The mice's body weight monitoring, blood tests, and pathological sections of major organs indicated good biocompatibility for MSNs of different sizes. Animal welfare and the animal experimental protocols were strictly consistent with related ethics regulations of Zhejiang Chinese Medical University. Overall, this study prepared MSNs with a particle size gradient to investigate the correlation between toxicity and particle size using macrophages and endothelial cells. The study also examined the biosafety of MSNs with different particle sizes in vivo and in vitro, which could help to improve the safety design strategy of MSNs for drug delivery systems.
