Icaritin and pyropheophorbide-a self-assembled nanomedicine for enhanced the efficacy of photodynamic tumor therapy by increase the cell autophagy
10.16438/j.0513-4870.2022-0997
- VernacularTitle:淫羊藿素与焦脱镁叶绿酸-a自组装纳米药物通过诱导细胞自噬促进肿瘤光动力疗效的研究
- Author:
Run-tian GUAN
1
;
Rong-rong ZHENG
1
;
Ni YANG
2
;
Xiao-na RAO
1
;
Shi-ying LI
1
Author Information
1. School of Pharmacy, Guangzhou Medical University, Guangzhou 511436, China
2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
photodynamic therapy;
autophagy;
self-assembly;
nanomedicine;
cancer
- From:
Acta Pharmaceutica Sinica
2023;58(8):2483-2493
- CountryChina
- Language:Chinese
-
Abstract:
Autophagy often occurs after cells are attacked by oxidative stress, where damaged structures are phagocytic and degraded into nutrients, thereby reducing oxidative damage, promoting the survival of cancer cells and reducing the therapeutic effect of photodynamic therapy (PDT). However, excessive activation of autophagy can promote cell apoptosis. In this paper, the photosensitizer pyropheophorbide-a (Ppa) was used to produce a large amount of reactive oxygen species (ROS) to achieve the effect of killing cancer cells. At the same time, icaritin (Ica), an autophagy inducer, was used to over-activate autophagy, which transformed the protection of cancer cells into the promotion of cancer cell apoptosis, so as to improve the effect of photodynamic therapy. In this study, the interaction force between Ica and Ppa was exploited to successfully construct a self-assembled nanomedicine IP with good stability and high drug load. The synthesis method is simple, through using the drug itself as a carrier, and the loading capacity (LA) of Ica and Ppa can be increased to 83.53% and 16.45% without introducing potential biosafety risks of nanocarriers. Compared with free Ppa, self-assembled nanomedicine IP showed superior performance in cellular uptake and reactive oxygen species production. In addition, the self-assembled nanomedicine IP can reverse the protective autophagy induced by PDT by activating the autophagy of tumor cells, and facilitate apoptosis and antitumor coordination, which significantly improves the antitumor activity of PDT.
