Analysis of the of Pb, Cd and As in decoction of Lindera aggregata (Sims) Kosterm. by PBET digestion in vitro/Caco-2 cell model and their cumulative risk assessment
10.16438/j.0513-4870.2022-0888
- VernacularTitle:基于体外消化/Caco-2细胞模型分析乌药水煎液中铅、镉和砷的生物可给量及累积风险评估
- Author:
Tian-tian ZUO
1
;
Ya-qiong SUO
1
;
Fei-ya LUO
1
;
De-juan KONG
2
;
Hong-yu JIN
1
;
Lei SUN
1
;
Shu-xia XING
1
;
Yuan-sheng GUO
1
,
3
;
Gang-li WANG
1
;
Shuang-cheng MA
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China
2. Tongliao Market Detection and Testing Center, Tongliao 028000, China
3. China Pharmaceutical University, Nanjing 211198, China
- Publication Type:Research Article
- Keywords:
italic>Lindera aggregata (Sims) Kosterm.;
ecoction;
PBET digestion in vitro/Caco-2 cell model;
bioaccessible content;
heavy metal and harmful element;
co-exposure;
cumulative risk assessment
- From:
Acta Pharmaceutica Sinica
2023;57(8):2461-2467
- CountryChina
- Language:Chinese
-
Abstract:
Inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine the concentrations of lead (Pb), cadmium (Cd) and arsenic (As) in Lindera aggregata (Sims) Kosterm. The physiologically based extraction test (PBET) digestion in vitro/Caco-2 cell model was established to investigate the bioaccessible contents of Pb, Cd and As in decoction of Lindera aggregata (Sims) Kosterm. The target-organ toxicity dose modification of HI method (TTD) was used to evaluate the cumulative risk caused by the combined exposure of the total levels of Pb, Cd and As in Lindera aggregata (Sims) Kosterm. and the bioaccessible contents in the decoction. The results showed that the total contents of Pb, Cd and As in 4 batches of samples were in the range of 2.901-3.872, 1.299-1.800 and 0.062-0.216 mg·kg-1, respectively. After transportation by Cacco-2 cells, the bioaccessible contents of Pb, Cd, and As in the decoction were in the range of 0.045-0.080, 0.070-0.112 and 0.004-0.018 mg·kg-1. The results of risk assessment showed that calculated by the total amounts of heavy metals in the Lindera aggregata (Sims) Kosterm., for the end points of nervous system, the cumulative risks of co-exposure of heavy metals in 3 batches of samples were of concern. After decoction and transportation by Caco-2 cells, for the end points of cardiovascular system, blood, nervous system, kidney and testis, the TTD modification of HI values of all batches of samples were less than 1, and the health risks were acceptable. The study provided methodology basis for a more objective assessment of the health risks of heavy metals and harmful elements in traditional Chinese medicine and for a more scientific limit standard of heavy metals and harmful elements.
