Mechanism of Zhibai Dihuang decotion in treating postmenopausal osteoporosis
10.16438/j.0513-4870.2023-0574
- VernacularTitle:知柏地黄汤治疗绝经后骨质疏松的机制研究
- Author:
Man-ya JIANG
1
;
Yue YE
1
;
Qiong-yi ZHANG
1
;
Gan-qing LUO
1
;
Wen-zhe DENG
1
;
Meng WANG
1
;
Kurihara HIROSHI
1
;
Jian-bo HE
1
,
2
,
3
,
4
;
Yi-fang LI
1
;
Rong-rong HE
1
,
2
Author Information
1. Guangdong Engineering Research Center of Chinese Medicine and Disease Susceptibility, Jinan University, Guangzhou 510632, China
2. School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, China
3. Integration of Traditional Chinese and Western Medicine, Jinan University, Guangzhou 510632, China
4. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China
- Publication Type:Research Article
- Keywords:
postmenopausal osteoporosis;
oxidative stress;
osteoclast;
bone resorption;
Zhibai Dihuang decoction
- From:
Acta Pharmaceutica Sinica
2023;58(10):3040-3048
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the ovarian surgery (ovariectomy, OVX) was used to establish the osteoporosis mice model of primary menstruation, in order to evaluate the protective effects and mechanisms of Zhibai Dihuang decotion on postmenopausal osteoporosis (PMOP). The animal experimental protocol has been reviewed and approved by Laboratory Animal Ethics Committee of Jinan University (number: 20210315-03), in compliance with the Institutional Animal Care Guidelines. C57BL/6 mice were divided into five groups, including Sham group, OVX group, low (32 g·kg-1·day-1) and high dose (64 g·kg-1·day-1) of Zhibai Dihuang decotion groups, positive drug group (alendronate, 9.9 mg·kg-1·q3d). After modeling, mice were given medication intervention for 8 weeks, and then femoral and tibial tissues were taken to detect indicators such as bone microstructure, bone resorption, and oxidative stress. The experimental results showed that after Zhibai Dihuang decotion administration, the bone microstructure damage caused by OVX surgery was alleviated, and the relevant parameters bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular number (Tb. N) and connectivity density (Conn. D) both significantly increased. At the same time, the number of TRAP positive osteoclasts decreased significantly, and the levels of proteins and genes related to osteoclast differentiation decreased, indicating that Zhibai Dihuang decoction could inhibit the increased activity of osteoclast caused by OVX. Afterwards, network pharmacology was used to construct the active compound action target network of Zhibai Dihuang decotion, and it was found that the target genes of its active ingredients were closely related to the oxidative stress pathway. Finally, the detection results of oxidative stress levels in bone tissues showed that after treatment with Zhibai Dihuang decotion, the levels of oxidative stress products 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) in bone tissues of mice significantly decreased, while the levels of antioxidant stress substance L-glutathione (GSH) increased. These above results indicated that Zhibai Dihuang decotion can regulate the level of oxidative stress in the body and inhibit osteoclast activity, which played a therapeutic role in PMOP, as well as provided theoretical basis for the prevention and treatment of PMOP with traditional Chinese medicine.
