DNA molecular identification of Polyrhachis dives medicinal materials
10.16438/j.0513-4870.2023-0269
- VernacularTitle:拟黑多刺蚁药材DNA分子鉴定研究
- Author:
Li-dan HE
1
;
Kai-jun ZHAO
2
;
Yun PENG
3
;
Fang HUANG
1
;
Hai-li WANG
2
Author Information
1. School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
2. Nanjing Zhongshan Pharmaceutical Co., Ltd., Jiangsu Classical Prescriptions Engineering Research Center, Nanjing 210046, China
3. Jiangsu Hongdian Research Institute of Traditional Chinese Medicine Industry Co., LTD., Nanjing Clinical Experience Prescription Modernization Engineering Research Center, Nanjing 210042, China
- Publication Type:Research Article
- Keywords:
italic>Polyrhachis vicina Roger;
italic>Polyrhachis dives;
DNA molecular identification;
specific PCR primers;
italic>CO Ⅰ gene
- From:
Acta Pharmaceutica Sinica
2023;58(10):3140-3146
- CountryChina
- Language:Chinese
-
Abstract:
In the study, specific primers were designed based on the CO Ⅰ gene sequence of Polyrhachis dives. By optimizing the genomic DNA extraction method and amplification conditions, we established an efficient, specific, and accurate DNA molecular identification method for Polyrhachis dives. In this method, the length of the target fragment was 294-308 bp, and the other counterfeits had no target bands. In this paper, the specific identification method of the origin of Polyrhachis dives established can be used to identify the medicinal materials of Polyrhachis dives accurately.
