The effects of natural polysaccharide supramolecular hydrogel on bacteriostatic activity and biofilm clearance of berberine
10.16438/j.0513-4870.2023-0165
- VernacularTitle:天然多糖超分子水凝胶对黄连素抑菌活性及生物膜清除能力的影响
- Author:
Yao-zhi ZHANG
;
Xue-mei HUANG
;
Ji-hui LU
;
Xiao-yu LIN
;
Lin-ying WU
;
Hai-min LEI
;
Peng-long WANG
- Publication Type:Research Article
- Keywords:
berberine;
natural polysaccharide;
supramolecular;
hydrogel;
biological activity
- From:
Acta Pharmaceutica Sinica
2023;58(10):3099-3107
- CountryChina
- Language:Chinese
-
Abstract:
Taking berberine (BBR) as an example, to study whether the supramolecular hydrogel formed by berberine and lotus root starch (Nelumbo nucifera Gaertn; LRS), a natural polysaccharide, affects the inhibition to Staphylococcus aureus and the ability of biofilm clearance. The chemical structure and rheological properties of BBR@LRS gel were characterized by infrared spectroscopy and rheometer. The in vitro release of supramolecular hydrogel was observed at pH = 1.2 and pH = 7.4. Broth dilution method and biofilm clearence experiment were used to observe the bacteriostasis and biofilm clearance respectively. Cytotoxicity test and in vitro hemolysis test were used to evaluate the biosafety preliminarily. The results showed that the LRS polysaccharide hydrogel could encapsulate BBR, and there was an interaction between them. The BBR@LRS gel had good rheological properties and biosafety, and played a role in solubility enhancement and slow release of BBR, which was stronger than BBR in inhibiting the growth of Staphylococcus aureus and clearing biofilm. This study provides reference for the effect of natural polysaccharide supramolecular hydrogels on biological functions of active components of traditional Chinese medicine.
