Astragalin Inhibits Apoptosis of Cerebral Cortex Neurons in APP/PS1 Mice
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0612
- VernacularTitle:紫云英苷抑制APP/PS1小鼠大脑皮质神经元凋亡
- Author:
Shu-han WANG
1
;
Cui-zhu YANG
1
;
Run-heng ZHANG
1
;
Jia-hong LIN
1
;
Ya-qi YANG
1
;
Jing LIU
1
;
Guo-ying LI
1
;
Yu-xin MA
1
Author Information
1. Department of Human Anatomy， Histology and Embryology， School of Life Sciences and Biopharmaceutics， Guangdong Pharmaceutical University， Guangzhou 510006， China
- Publication Type:Journal Article
- Keywords:
astragalin （AST）;
APP/PS1 mice;
cerebral cortex;
neuron;
apoptosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(6):983-990
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Astragalin （AST） on apoptosis of cerebral cortex neurons in APP/PS1 transgenic mice. MethodsEighteen six-month-old male APP/PS1 transgenic mice were randomly divided into APP/PS1 group， APP/PS1+ 40 mg/kg AST group and APP/PS1+ 20 mg/kg Donepezil （DNP） group， with six mice in each group. At the same time， six male C57BL/6 mice were selected as the normal control group. After intraperitoneal injection of AST once a day and continuous administration for one month， we used Tunel staining to detect the apoptosis of neurons in the cerebral cortex of APP/PS1 mice； immunofluorescent staining to examine the expression of apoptosis-related proteins Bax， Bcl-2， Caspase9 and Cleaved-Caspase3 in the cerebral cortex neurons of APP/PS1 mice； Western blot method to evaluate the changes of the expression of Bax， Bcl-2， Caspase9 and Caspase3. ResultsTunel staining showed that 40 mg/kg AST and 20 mg/kg DNP both reduced the apoptosis of neurons in the cerebral cortex of APP/PS1 mice， AST with more significant inhibition effect. Immunofluorescent staining revealed that 40 mg/kg AST and 20 mg/kg DNP both inhibited the expression of Bax， Caspase9， and Cleaved-Caspase3， and icreased the expression of Bcl-2 in the cerebral cortex neurons of APP/PS1 mice. Western blot results further confirmed that 40 mg/kg AST and 20 mg/kg DNP both down-regulated the expression of Bax （P < 0.05， P < 0.05）， Caspase9 （P < 0.005， P < 0.05） and Caspase3 （P < 0.0001， P < 0.0001） ， and up-regulated the expresstion of Bcl-2 （P < 0.05， P < 0.05） in the cerebral cortex neurons of APP/PS1 mice. ConclusionsAST can inhibit the apoptosis of cerebral cortex neurons in APP/PS1 mice.
