Human Gingival Stem Cells Prevent Diabetes in NOD Mice by Reducing Follicular B Cells
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0609
- VernacularTitle:人牙龈间充质干细胞通过减少滤泡B细胞比例预防非肥胖糖尿病小鼠糖尿病
- Author:
Yi-wen GUO
1
;
Bao-ning LIU
2
;
Jia YU
1
;
Xin-hua CHANG
1
;
Mao-gen CHEN
1
;
Yi MA
1
;
Su-xiong DENG
1
;
Rong-hai DENG
1
Author Information
1. Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
2. The First Clinical School of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510080, China
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cell;
type 1 diabetes;
follicular B cell;
NOD mice;
blood glucose
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(6):958-964
- CountryChina
- Language:Chinese
-
Abstract:
BackgroundType 1 diabetes is caused by a chronic immune response that destroys islet beta cells， resulting in elevated blood glucose. Mesenchymal stem cells can prevent and treat the development of diabetes and its complications. However， little is known about the effects and potential mechanisms of Gingival mesenchymal stem cells （GMSCs） in preventing diabetes. The aim of this study is to investigate the mechanism of GMSCs in preventing type 1 diabetes in mice and to find targets for clinical treatment of diabetes. MethodsWe injected human GMSCs into NOD mice to observe the trend of blood glucose， observed the survival of pancreatic β-cells by immunohistochemistry， and detected the change of immune cells in the spleen of mice by flow analysis. Finally， the immune cells in NOD mice were transfused into NOD-SCID mice to observe the onset of diabetes in NOD-SCID mice. ResultsGMSCs significantly reduced the incidence of diabetes in NOD mice， with 64% of control mice developing diabetes at 27 weeks of age compared with 35% in the GMSC group， P=0.013. The percentage of Follicular B cells（FO B cell） in the spleen of GMSCs-treated mice decreased from （52.2±4.1）% to （43.2±5.3）%， P=0.008， while other types of immune cells did not change significantly. The immunohistochemical results showed that GMSCs could effectively improve the survival of pancreatic β-cells， which could continuously produce insulin to control blood glucose. Finally， we found the spleen cells transfusion could prevent the development of diabetes in NOD-SCID mice. ConclusionGMSCs can reduce diabetes in mice by reducing FO B cells in the spleen.
