Cytotoxic Activity of Ethanolic Extract Aquilaria malaccensis Leaves Against MCF-7 Cells
https://doi.org/10.47836/mjmhs.19.6.29
- Author:
Muhammad Yusran Abdul Aziz
1
;
Syed Ahmad Tajudin Tuan Johari
2
,
3
;
Wan Nur Amalina Wan Mamat
3
;
Wan Rohani Wan Taib
4
;
Ahmad Syibli Othman
4
;
Mohd Adzim Khalili Rohin
4
Author Information
1. UniSZA Science and Medicine Foundation Centre, Universiti Sultan Zainal Abidin, Gong Badak Campus, 21300 Kuala Nerus, Terengganu, Malaysia
2. Centre for Research in Infectious Disease and Biotechnology, Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, 20400 Kuala Terengganu, Terengganu, Malaysia&
3. Centralised Lab Management Centre, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia
4. Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Gong Badak Campus, 21300 Kuala Nerus, Terengganu, Malaysia
- Publication Type:Journal Article
- Keywords:
Aquilaria malaccensis, breast cancer, MCF-7, cytotoxicity, apoptosis, mitochondria membrane potential
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.6):215-221
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Aquilaria malaccensis, also known as “Pokok Karas” in Malaysia, is widely used in Southeast Asian
countries for the treatment of joint pain, diarrhoea and inflammatory diseases, and has shown beneficial effects as
an anticancer agent. The aim of this study was to investigate the effect of ethanol leaf extracts of A. malaccensis on
MCF-7 cells. Methods: MTT-based cytotoxic and antiproliferative assay was used to determine the outcome of ethanolic extract toward MCF-7 cells. The mode of cell death was determined by the AO/PI double staining assay and
the depolarisation of the mitochondria membrane potential. Results: IC50 value of the extract against MCF-7 cells
treated for 72 hours was 4.1 ± 2.08 µg/mL, while the IC50 value for doxorubicin was 2.92 ± 0.12 µg/mL. The extract
showed a lower cytotoxic effect against the NIH/3T3 cells and inhibited the growth of MCF-7 cells in a dose dependent manner. AO/PI double stain showed that the ethanolic extract of A. malaccensis leaves induced MCF-7 cells
into apoptotic cell death. The present study showed that the ethanolic extract of A. malaccensis induced apoptosis
through mitochondrial pathway as indicated by its ability to take up JC-1. Conclusion: The study found that ethanolic
extract obtained from A. malaccensis leaves is cytotoxic on MCF-7 cells, resulting to apoptotic cell death of the cells.
- Full text:11.2023my1683.pdf
