Impacts of isorhynchophylline on airway inflammation in asthmatic mice
- VernacularTitle:异钩藤碱对哮喘小鼠气道炎症的影响
- Author:
Jin CAI
1
;
Zhaozhao HUA
2
;
Changrong ZHANG
2
;
Dan HUANG
1
;
Qihua ZHANG
1
;
Yi WANG
1
;
Sufang ZHOU
3
;
Lian LIU
4
;
An GONG
5
Author Information
1. Dept. of Classic TCM，the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang 550001，China
2. Dept. of Obstetrics，the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang 550002，China
3. Research Center of Microecology，Guizhou University of Traditional Chinese Medicine，Guiyang 550001，China
4. Clinical Microbiology and Immunology Teaching and Research Office，Guizhou Medical University，Guiyang 550001，China
5. Qihuang Academy of Traditional Chinese Medicine，Jiangxi University of Traditional Chinese Medicine，Nanchang 330025，China
- Publication Type:Journal Article
- Keywords:
isorhynchophylline;
asthma;
monocyte chemotactic protein-1/CC chemokine receptor 2 signaling pathway;
airway
- From:
China Pharmacy
2023;34(22):2740-2744
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the impacts of isorhynchophylline （IRN） on airway inflammation in asthmatic mice by regulating the monocyte chemotactic protein-1 （MCP-1）/CC chemokine receptor 2 （CCR2） signaling pathway. METHODS The asthmatic mice model was established by injecting and inhaling ovalbumin. The successfully modeled mice were randomly grouped into asthma group， IRN low-dose group （IRN-L， intragastric administration of 10 mg/kg IRN）， IRN high-dose group （IRN-H， intragastric administration of 20 mg/kg IRN）， IRN-H+CCL2 group [intragastric administration of 20 mg/kg IRN+intraperitoneal injection of 7.5 ng CC chemokine ligand 2 （CCL2）] and positive control group （intraperitoneal injection of 2 mg/kg dexamethasone）. The mice injected and inhaled with sterile phosphate-buffered solution were included in the blank control group， with 10 mice in each group. The mice in administration groups were given relevant medicine once a day， for consecutive 2 weeks. The levels of airway hyperreactivity indexes such as enhanced （Penh） value， tumor necrosis factor-α （TNF-α），interleukin-13 （IL-13） and IL-4 in serum， the number of eosinophil （EOS）， lymphocyte （LYM） and neutrophils （NEU） in alveolar lavage fluid and the protein expressions of MCP-1 and CCR2 in lung tissue were observed in each group； the pulmonary histopathological changes were observed， and inflammatory cell infiltration score was evaluated. RESULTS Compared with the blank control group， the infiltration of inflammatory cells in the lung tissue of mice was more significant in the asthma group， and there was swelling and shedding of cells； inflammatory infiltration score， Penh value， the levels of IL-4， IL-13 and TNF-α， the number of EOS， NEU and LYM， the protein expressions of MCP-1 and CCR2 were increased significantly （P＜0.05）. Compared with the asthma group， the pathological injuries of the IRN-L group， IRN-H group and positive control group were improved， and the above quantitative indexes were decreased significantly （P＜0.05）. Compared with the IRN-L group， the above quantitative indexes of the IRN-H group and positive control group were decreased significantly （P＜0.05）. There was no statistical significance in the above quantitative indexes between the IRN-H group and the positive control group （P＞0.05）. Compared with the IRN-H group， the above quantitative indexes of the IRN-H+CCL2 group were increased significantly （P＜0.05）. CCL2 reversed the protective effect of high-dose IRN on asthmatic mice. CONCLUSIONS IRN may reduce the release of airway inflammatory factors in asthmatic mice by inhibiting the activation of the MCP-1/CCR2 signaling pathway， so as to achieve the purpose of improving asthma.
