Determination of bongkrekic acid in corn flour by direct extraction ultra-high performance liquid chromatography tandem mass spectrometry
10.3969/j.issn.1006-2483.2023.06.018
- VernacularTitle:直接提取法-超高效液相色谱串联质谱法测定玉米面粉中米酵菌酸含量
- Author:
Yuan WANG
1
;
Chunlin BAI
1
;
Jiheng LIU
2
Author Information
1. Heilongjiang Center for Disease Control and Prevention , Harbin , Heilongjiang 150030 , China
2. Yichang Center for Disease Control and Prevention , Yichang , Hubei 443000 , China
- Publication Type:Journal Article
- Keywords:
Bongkrekic acid;
Direct extraction;
Liquid chromatography-tandem mass spectrometry;
Corn flour;
Solid phase extraction
- From:
Journal of Public Health and Preventive Medicine
2023;34(6):76-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a direct extraction ultra-high performance liquid chromatography tandem mass spectrometry method for the determination of bongkrekic acid in corn flour. Methods Bongkrekic acid was directly extracted with 80% methanol from corn flour samples, and the supernatant after vortex and centrifugation was determined after passing through membrane filtration. At the same time, the corn flour samples were extracted by solid phase extraction. The determination results of the two methods were compared. Results The linearity of standard series was good within the range of2-20 μg/L, and the linearity coefficient was＞0.999. The determination result of the positive sample by direct extraction method was 193.40 mg/kg (n=6). Adding the standard to the blank sample at the levels of 2, 6, and 10 μg/L, the calculated recovery rate was 75.82% - 99.33%, and the relative standard deviation was 3.54 % - 8.45%. The detection limit of the method reached 6 μg/kg. After extraction by solid phase extraction, the determination result of the positive sample was 196.84 mg/kg (n=6). The recovery rate was 77.12% -100.83%, with a relative standard deviation of 8.32% - 9.54%. Conclusion Compared with the solid phase extraction, the direct extraction method for the extraction of bongkrekic acid from corn flour has the advantages of rapidity, simplicity, and cost savings.
