TLC identification and quantitative determination of Shipi Xiaoshui gel plaster
10.12206/j.issn.2097-2024.202205054
- VernacularTitle:实脾消水凝胶贴膏的薄层色谱鉴别和含量测定研究
- Author:
Bingzhi MA
1
;
Yingying LIANG
1
;
Haiyang WANG
1
;
Yonghe TANG
1
;
Dong LI
1
;
Jun HE
1
Author Information
1. Department of Pharmacy, China Japan Friendship Hospital, Beijing 100029, China.
- Keywords:
Shipi Xiaoshui gel plaster;
quality standard;
thin layer chromatography;
UPLC-MS;
astragaloside Ⅳ
- From:
Journal of Pharmaceutical Practice
2023;41(11):662-665
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of thin layer chromatography (TLC) for identification and quantitative determination of Shipi Xiaoshui gel plaster. Methods TLC was adopted to qualitatively identify astragalus radix, plantaginis semen, curcumae rhizome, cinnamomi ramulus, polyporus umbellatus and akebia quinata. UPLC-MS was used to determine the content of astragaloside Ⅳ. Results TLC spots were clear and well-separated; RSDs of precision, reproducibility and stability tests were all lower than 3%, the linear range of astragaloside Ⅳ was 2.75-33 μg/ml (r=0.999 9, n=6), and the average recovery was 100.49% (RSD=1.98%, n=6). Conclusion The established method in this study is accurate, reliable and specific, which could be used for the quality control of Shipi Xiaoshui gel plaster.
