Effects and mechanisms of curcumin on apoptosis of retinal ganglion cells in chronic ocular hypertension rats
10.3980/j.issn.1672-5123.2023.12.02
- VernacularTitle:姜黄素对慢性高眼压大鼠视网膜神经节细胞凋亡的影响及机制
- Author:
Wan-Jing XU
1
;
Yu-Hao SUN
1
;
Shuang ZHAO
1
;
Jun ZHAO
1
;
Juan-Mei ZHANG
1
Author Information
1. Department of Ophthalmology,QingPu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201799, China
- Publication Type:Journal Article
- Keywords:
curcumin;
Spraque-Dawley rats;
chronic ocular hypertension model;
retinal ganglion cells;
apoptosis;
glaucoma
- From:
International Eye Science
2023;23(12):1943-1949
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects and mechanisms of curcumin on apoptosis of retinal ganglion cells(RGCs)in chronic ocular hypertension rats.METHODS:A total of 21 Spraque-Dawley(SD)rats were randomly divided into 3 groups with 7 rats in each group. The rat models of chronic ocular hypertension were established by cauterization of the superior scleral veins in the high intraocular pressure model group and the curcumin treatment group, and the sham operation group only cut the conjunctiva without the cauterization of the superior scleral veins; the rats in the curcumin treatment group were intragastrically treated with curcumin at a dose of 4mL/kg, and the rats in the sham operation group and the high intraocular pressure model group were treated with pure water at a dose of 4mL/kg for 3wk. After 3wk, HE staining was used to observe the morphological and pathological changes of retina, the number of RGCs and the thickness of ganglion cell layer(GCL)in each group of rats; TUNEL staining was used to observe the apoptosis of RGCs and retinal cells in each group of rats; the expression levels of glutamate-cysteine ligase modifier subunit(GCLM)and heme oxygenase-1(HO-1)in the retina of each group of rats were detected by real-time fluorescence quantitative PCR, immunohistochemical staining and Western blot.RESULTS:Compared with the sham operation group, the retinal morphology of rats in the high intraocular pressure model group and the curcumin treatment group was disorganized, the number of RGCs was reduced, the GCL was thinner, the apoptosis rate of RGCs and retinal cells increased, and the expression levels of GCLM and HO-1 increased. Compared with the high intraocular pressure model group, the retinal morphology of rats in the curcumin treatment group was basically normal, the number of RGCs increased, the GCL thickened, the apoptosis rate of RGCs and retinal cells decreased, and the expression levels of GCLM and HO-1 increased.CONCLUSION:Curcumin can inhibit the apoptosis of RGCs in the rat model of chronic ocular hypertension by up-regulating the expression of antioxidant genes GCLM and HO-1.
