Phytochemicals Screening and Anti-proliferative Activities of Anacardium occidentale Shoot Extract in Breast Cancer Cells
https://doi.org/10.47836/mjmhs.19.5.29
- Author:
Pui Kee Chan
1
;
Nurul Husna Shafie
1
,
2
;
Mohd Amir Ashraf Meli
1
;
Su Peng Loh
1
Author Information
1. Department of Nutrition, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia&
2. Laboratory of UPM-MAKNA Cancer Research, Institute of Bioscience UPM, 43400 Serdang, Malaysia
- Publication Type:Journal Article
- Keywords:
Anacardium occidentale, Shoot, Anticancer, Apoptosis, Phytochemicals
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.5):202-210
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Anacardium occidentale or cashew are popular traditional food and have raised research interest
for complementary cancer treatment. Cancer has become leading cause of death and treatment involved severe
side effects. In present study, we aim to study the anti-proliferative effects of cashew shoots in breast cancer (MDAMB-231), colorectal cancer (HT-29) and liver cancer (HepG2) cell lines. Methods: Cell lines were treated with 70%
ethanolic cashew extract for cytotoxicity test with MTT assay. AO/PI dual fluorescent assay and RNase/PI staining
were used to determine apoptosis induction effects. Phytochemicals screening was carried out by using gas chromatography mass spectrometry (GCMS) and liquid chromatography mass spectrometry (LCMS). Results: The cytotoxicity assay of cashew shoot extract demonstrated IC50 of 81.1 ± 0.11 μg/ml for MDA-MB-231, 307.5 ± 2.31 μg/ml
for HT-29 and 272.6 ± 1.91 μg/ml for HepG2 cell lines. The apoptotic bodies include chromatin condensation, cell
blebbing and nuclear fragmentation and apoptosis induction were shown by AO/PI staining. There was significant
increase of cell count in sub-G0 phase in MDA-MB-231 cell lines treated with cashew shoot extract. It was demonstrated that cashew shoot extract contained 38 compounds from GCMS such as sitosterol, tannin, pyrogallol, phenol
and 20 compounds from LCMS such as citric acid, gallic acid, myricetin and hinokiflavone that may give rise to its
anti-cancer effect. Conclusion: Cashew shoot extract demonstrated potential anti-cancer properties thus further study
is required to investigate its mechanism as anti-cancer agent.
- Full text:11.2023my1631.pdf
