Glutamate Involved in Rats with Spinal Cord Injury Induced by Dynorphin A (1-17)
- VernacularTitle:强啡肽致大鼠脊髓损伤与谷氨酸能神经功能关系的研究
- Author:
Fuchun LI
;
Pingping ZUO
;
Wenhui HU
;
Na LIU
;
Minfeng REN
- Publication Type:Journal Article
- Keywords:
spinal cord injury, dynorphin, glutamate release, NMDA receptor
- From:
Chinese Journal of Rehabilitation Theory and Practice
1997;3(1):6-9
- CountryChina
- Language:Chinese
-
Abstract:
Intrathecal (I. T.)administration of K opioid dynorphin A (1-17) is used as a model of neurological dysfunction which lnvolved in spinal cord injury and secondary affection according to several previous reports. 5-amlno-2-phosphoveroleric acid (APV ), an NMDA receptor antagonist, can significantlly prevent the hindlimb paralysis in rats produced by I. T. dynorPhin A (1-17). To further investigate the mechanisms, we establis11 a nlodel of [3H]L,-Glu release in rats spinal slices influenced by dynorphin A (dynA ) (1-17). [3H]L-Glu release evoked by high [K+] (5Ommol/L,)is a Ca2+-dependent process. DynA (1-17) slgnificantly inhibited [3H]L,-Glu release at 1O-8mol/L,, but very significantly enhanced [3H]L-Glu release at 10-6 mol /L. The synthetic k agonist U50, 488H, which has no neurotoxic effect, inhibited [3H]L-Glu release at both high and low concentrations and did not have any enhancing effect. The results suggest that the analgesic effect of dynA (1-17) at physiological dosage may be rnediated by presynaptic K opioid receptor through the inhibition of Ca2+ influx and L-Glu release;but dynA (1-l7)enhanced L-Glu release through a non-opioid pathway and induced hindlimb paralysis at high neurotoxic dosage.
