Subchronic Administration of High-dose Monosodium Glutamate Causes Spatial Memory Dysfunction and Structural Deficits of Rat Hippocampus
https://doi.org/10.47836/mjmhs.19.4.24
- Author:
Muchtar Hanafi
1
;
Faizal Muhammad
2
;
Amilia Ramadhani
3
;
Nurlaela
4
;
Zaenal Muttaqien Sofro
5
;
Ginus Partadiredja
5
Author Information
1. UNS Hospital, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia, 57161
2. Neurology Department, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia 57126
3. School of Dentistry, Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Indonesia 53122
4. Faculty of Medicine, Nursing, and Public Health, Gadjah Mada University, Yogyakarta, Indonesia 55281
5. Physiology Department, Faculty of Medicine, Nursing, and Public Health, Gadjah Mada University, Yogyakarta, Indonesia 55281
- Publication Type:Journal Article
- Keywords:
Cognitive, Hippocampus, Monosodium glutamate, Spatial memory, Stereology
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.4):159-165
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: The toxicity of high concentration monosodium glutamate (MSG) has become a controversial issue
because of its inconsistent results in human and animal studies. This present study aims to evaluate the effect of subchronic high-doses oral administration of MSG on spatial memory performance and hippocampal pyramidal cells
number. Methods: This study involved twenty-eight male Wistar rats, which were divided into a control group of
NaCl 0.9% and three intervention groups of MSG 1.0 mg/g bodyweight (M1), 2.0 mg/g bodyweight (M2), and 4.0
mg/g bodyweight (M3) for 30 days. Statistical analysis used a One-way ANOVA test. Results: The result showed significant differences in spatial memory on the Morris Water Maze (MWM) test, including path length (p = 0.020) and
escape latency (p = 0.011) according to general linear model repeated measurement analysis. The mean difference
of estimated hippocampal pyramidal cells total number among the groups showed volume (p = 0.001), numerical
density (p = 0.590), and cells number (p = 0.004). Furthermore, Post-Hoc analysis in both spatial memory and hippocampal pyramidal cells showed that the increasing MSG dose from 1.0 to 4.0 mg/g bodyweight led to a decrease in
the results of spatial memory performance on the MWM test and a decrease in hippocampal cells. Conclusion: The
present study has provided novel quantitative data that subchronic administration of high-dose MSG caused deleterious effects on the spatial memory function and the volume and number of hippocampal pyramidal cells.
- Full text:11.2023my1575.pdf
