The Effects of Glucocorticoid and alpha-Lipoic Acid on Ischemia-Reperfusion Injury.
- Author:
Sang Soon LEE
1
;
Yoon Jae CHUNG
;
Byung Kyu SOHN
;
Hyung Geon KIM
;
Seong Ha PARK
Author Information
1. Department of Plastic and Reconstructive Surgery, Dankook University, College of Medicine, Seoul, Korea. prslee@hananet.net
- Publication Type:Original Article
- Keywords:
Ischemia-reperfusion injury;
Glucocorticoid;
alpha-Lipoic acid
- MeSH:
Animals;
Arachidonic Acid;
Edema;
Free Radicals;
Ischemia;
Metabolism;
Muscle, Skeletal;
Neutrophils;
Oxygen;
Rats;
Reperfusion;
Reperfusion Injury*;
Thioctic Acid*;
Tourniquets
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2002;29(3):205-210
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
During revascularization after ischemia, oxygen free radicals and cytotoxic enzymes are released and they have a role in pathogenesis of ischemia-reperfusion injury. Glucocorticoid decreases oxygen free radical formation by inhibition of arachidonic acid metabolism, and alpha-lipoic acid scavenges nitric oxide(NO) with inhibition of hydroxy radical formation. Author investigated the role of glucocorticoid and alpha-lipoic acid to decrease ischemia reperfusion injury in 24 anesthetized rats (normal saline-injected, n= 8; dexamethasone-injected, n=8; alpha-lipoic acid-injected, n= 8), subjecting a soleus muscle to 4 hours of tourniquet ischemia followed by 2 hours of reperfusion, and evaluated the concentration of NO, tissue edema, and neutrophil count of rat skeletal muscle as a indicator of tissue damage by ischemia- reperfusion injury. We obtained the results that glucocorticoid and alpha-lipoic acid treatment decreased the increase of NO concentration, tissue edema, and neutrophil count significantly. These results support that pretreatment with glucocorticoid or alpha-lipoic acid has a beneficial effect on the preventive management of ischemia-reperfusion injury.
