Determination of pentachlorophenol in wooden chopping boards with gas chromatography-tandem mass spectrometry
10.19485/j.cnki.issn2096-5087.2023.09.021
- Author:
ZHAO Yuwen
;
LI Zeran
;
XUE Xiangju
;
YANG Meng
;
JI Wenliang
;
RONG Weiguang
- Publication Type:Journal Article
- Keywords:
wooden chopping board;
pentachlorophenol;
gas chromatography-tandem mass spectrometry
- From:
Journal of Preventive Medicine
2023;35(9):825-828
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the determination of pentachlorophenol in wooden chopping boards through pretreatment of miniaturized samples.
Methods: The pretreated wooden chopping board samples were subjected to ultrasound extraction (1 mL of 0.5 mol/L K2CO3 added in 5 mL extraction solution) in 8 mL acetone and 2 mL water, followed by derivatization with 0.3 mL acetic anhydride, extraction with n-hexane and separation with DB-5ms column (30 m×0.25 mm, 0.25 μm). Gas chromatography tandem mass spectrometry (GC-MS/MS) was performed in multiple reaction monitoring (MRM) mode with quantitative analysis using the internal standard method.
Results:The GC-MS/MS assay showed a good linear relationship within the range of 0.01 to 0.2 µg/mL (R2>0.999), with a 0.003 mg/kg limit of detection and 0.01 mg/kg limit of quantitation. The mean recovery rates were 84.2% to 96.7% at spiked concentrations of 0.003, 0.01 and 0.03 mg/kg, with relative standard deviation of 2.2% to 6.1%.
Conclusions:The established GC-MS/MS assay is easy to perform, environment-friendly, highly accurate and sensitivity, which is feasible for determination of pentachlorophenol in wooden chopping boards.
- Full text:气相色谱串联质谱法检测木砧板中五氯苯酚.pdf
