Effects of lactoprotein iron chelates on iron deficiency anaemia in rats
10.19485/j.cnki.issn2096-5087.2023.10.007
- Author:
CHEN Jili
;
LI Minghui
;
WANG Mengying
;
XU Caiju
;
ZHANG Shixin
;
YAN Jun
;
PAN Wenfei
;
GAO He
;
LI Jie
;
WANG Hanbin
;
MA Yongqing
;
YANG Min
- Publication Type:Journal Article
- Keywords:
iron deficiency anaemia;
lactoprotein;
iron chelate;
liver function
- From:
Journal of Preventive Medicine
2023;35(10):861-865
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of lactoprotein iron chelates on rats with iron deficiency anaemia (IDA), so as to provide insights into developing and utilizing novel iron supplements.
Methods:Seventy weaning female SPF-graded rats of the SD strain were randomly divided into the control group (A), model group (B), ferrous sulfate group (C), lactoferrin group (D), lactoferrin iron chelate group (E), Casein oligopeptide iron chelate group (F) and whey protein oligopeptide iron chelate group (G), with 10 rats in each group. The rats in group A were fed with normal diet, and the others were fed with poor iron diet for IDA modeling. The corresponding interventions were given by intragastric administration once a day. The iron ion concentrations of group C, E, F and G were 2.0 mg/kg, and the protein and oligopeptide concentrations of group D, E, F and G were 2 000 mg/kg. Body weight and hemoglobin of rats were measured weekly during 21-day intervention. At the end, peripheral blood samples were collected, and blood routine, iron metabolism and liver function indicators were determined.
Results:After the intervention, among blood routine indicators, the rats in group C, E, F and G showed elevated hemoglobin, red blood cell, mean corpuscular volume and hematocrit, and decreased free protoporphyrin and mean corpuscular hemoglobin concentration when compared with the rats in group B (all P<0.05); among iron metabolism indicators, the rats in group C, E and G showed elevated serum ferritin, the rats in group C, E, F and G showed elevated serum iron, the rats in group C, D, E, F and G showed decreased unsaturated iron binding capacity and total iron binding capacity when compared with the rats in group B (all P<0.05); among liver function indicators, the rats in group E and G showed decreased alanine transaminase when compared with the rats in group B (both P<0.05).
Conclusions:Lactoprotein alone could not completely improve IDA in rats compared with traditional iron supplement (ferrous sulfate). Lactoprotein iron chelate, especially whey protein oligopeptide iron chelate, could significantly improve IDA, iron reserve and liver function damage in rats.
- Full text:乳蛋白铁螯合物对大鼠缺铁性贫血的影响研究.pdf
