Optimization of the formulation of a porcine fibrin patch
- VernacularTitle:猪源纤维蛋白贴配方的优化
- Author:
Rubing LI
1
;
Weida LI
2
;
Wenting LIU
1
;
Yulu LI
1
;
Xiaohong GU
1
;
Lijuan YU
1
;
Huayin WAN
3
Author Information
1. Dept. of Pharmacy，Shenzhen Hospital，University of Chinese Academy of Sciences，Guangdong Shenzhen 518107，China
2. Dept. of Pharmacy，Foshan Maternity and Child Healthcare Hospital，Guangdong Foshan 528000，China
3. Guangzhou Zhongwei Biotechnology Co.，Ltd.，Guangzhou 510010，China
- Publication Type:Journal Article
- Keywords:
porcine fibrin patch;
formulation optimization;
fibrinogen;
thrombin;
collagen;
Box-Behnken-response surface
- From:
China Pharmacy
2023;34(20):2497-2501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the formulation of a porcine fibrin patch （abbreviated as “DBT”）. METHODS Based on single-factor tests， with the contents of fibrinogen， thrombin and collagen before freeze-drying as the factors， with the overall desirability （OD） value of adhesion strength， holding viscosity and water absorption as response value， the formulation of DBT was optimized by Box-Behnken-response surface methodology， and the verification tests were conducted. RESULTS According to the results of the single factor tests and Box-Behnken-response surface methodology， combined with the actual production， the optimal formulation of DBT was 6.5 mg/cm2 of fibrinogen， 8.0 IU/cm2 of thrombin and 5.6 mg/mL of collagen. The average OD value of 3 validation tests was 0.726 6 （RSD＝0.58%， n＝3）， and the relative error of which with the predicted value （0.733 0） was －0.87%. CONCLUSIONS The optimal formulation of DBT is stable and feasible.
