Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora
- VernacularTitle:裸花紫珠醇提物UPLC指纹图谱建立及抗呼吸道合胞病毒谱效关系研究
- Author:
Bingmei SHAO
1
;
Xiaofeng LI
2
;
Beibei YAN
3
;
Qifeng LIU
4
;
Huanyun QIN
1
;
Xiangjun ZHAO
1
;
Lin HOU
1
Author Information
1. Qingdao Academy of Chinese Medicinal Sciences，Shandong University of Traditional Chinese Medicine，Shandong Qingdao 266000，China
2. National Enterprise Technology Center，Jiangxi Puzheng Pharmaceutical Co.，Ltd.，Jiangxi Jiʼan 343100，China
3. School of Pharmacy，Shandong University of Traditional Chinese Medicine，Jinan 250300，China
4. Dept. of Neurosurgery，Tai’an Municipal Hospital，Shandong Tai’an 271000，China
- Publication Type:Journal Article
- Keywords:
Callicarpa nudiflora;
ethanol extract;
respiratory syncytial virus;
fingerprint;
anti-virus;
spectrum-effect
- From:
China Pharmacy
2023;34(20):2465-2470
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the fingerprint of the ethanol extract from Callicarpa nudiflora， analyze its anti- respiratory syncytial virus （RSV） activity in vitro， and study the relationship between spectrum and effect. METHODS Using 10%， 30%， 50%， 70% and 90% ethanol as solvent， 20 batches of ethanol extracts from 4 batches of C. nudiflora were prepared. The fingerprints for 20 batches of ethanol extracts from C. nudiflora were mapped by ultra-high-performance liquid chromatography （UPLC）， and the similarity evaluation was conducted by using the Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints （2012 edition）. The cytopathic effect method and MTT method were used to investigate the in vitro inhibitory activity of the ethanol extracts from C. nudiflora on RSV. Pearson correlation analysis， grey correlation degree and orthogonal partial least squares （OPLS） analysis were used to study the spectrum-effect relationship. RESULTS There were 25 common peaks in 20 batches of ethanol extracts from C. nudiflora， and the similarities ranged from 0.912 to 0.998， and the RSDs of common peak areas were 33.54%-162.28%. The average values of IC50 for RSV of 20 batches of ethanol extracts from C. nudiflora were 9.55-272.23 μg/mL. The results of Pearson correlation analysis， grey correlation analysis and OPLS analysis showed that the Pearson correlation coefficients （P＜0.05） of the common peaks 8， 10， 12， 16， 18-19， 22-24 with pharmacodynamic indicators and regression coefficients were all negative， the correlation coefficients were all greater than 0.6， and the values of variable importance in projection were all greater than 1. CONCLUSIONS Twenty batches of ethanol extracts from C. nudiflora have similar components but significant differences in content， and exhibit different degrees of anti-RSV activity in vitro. The corresponding components of common peaks 8， 10， 12， 16， 18-19， 22-24 may be the characteristic components of anti-RSV of C. nudiflora.
