Expression of Stro-1, Runx-2, Osterix, and Alp in Alveolar Bone Regeneration Process Following the Administration of Hydroxyapatite Gypsum Puger (HAGP) Scaffold
https://doi.org/10.47836/mjmhs.19.3.19
- Author:
Amiyatun Naini
1
;
Agus Sumono
2
;
Rahardyan Parnaadji
1
;
Nike Hendrijantini
3
;
Siti Khaerunnisa
4
Author Information
1. Departement of Prostodontic, Faculty of Dentistry, University of Jember, Jember-Indonesia
2. Departement of Basic Dentistry, Faculty of Dentistry, University of Jember, Jember-Indonesia
3. Departement of Prostodontic, Faculty of Dentistry, Airlangga University, Surabaya- Indonesia
4. Departement of Physiology and Medical Biochemistry , Faculty of Medicine, Airlangga University, Surabaya- Indonesia
- Publication Type:Journal Article
- Keywords:
Stro-1, Runx-2, Osterix, Alp, Alveolar Bone Regeneration, Hydroxyapatite Gypsum Puger Scaffold
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.3):145-151
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Tooth extraction before denture placement could result in trauma and damage to up to 50% of the
alveolar bone, inducing bone resorption, and affecting the patient’s quality of life. Hydroxyapatite Gypsum Puger
(HAGP) can be used as an alternative to bone graft material which degrades slowly, affecting the proliferation and
activity of cells that are responsible for bone tissue engineering. This study aimed to analyze the regeneration mechanism of alveolar bone by administering the HAGP scaffold and observing the Stro-1, Runx-2, Osterix, and ALP
expression. Methods: Laboratory experimental research was conducted and we used 150-355µm HAGP scaffold
particles, applied in vivo inside alveolar sockets of the rats for 7, 14, and 28 days, followed by immunohistochemical
examination of Stro-1, Runx-2, Osterix, and ALP expressions. Results: The HAGP scaffold group showed that the
Stro-1 expression was significantly higher than the K(-) group, and the Runx-2 expression increased on day 7 and
decreased on day 28 in the HAGP and K(-) groups. Osterix expression increased from day 7, 14, to day 28. The high
expression of Osterix on day 28 means it took over the Runx-2 function. In ALP there was a significant increase on
day 7. ALP expression was a sign of early osteoblast differentiation and production by cells, this extracellular matrix
mineralization is an indicator of the osteogenic process. Conclusion: Alveolar bone regeneration mechanism in rats
revealed that the expression of Stro-1, Runx-2, Osterix, and ALP was higher in the HAGP scaffold group compared
to the control group on days 7,14, and 28.
- Full text:11.2023my1519.pdf
