Caspase 3 Expressions in Children with Acute Lymphoblastic Leukemia During Induction Phase Chemotherapy
https://doi.org/10.47836/mjmhs.19.3.12
- Author:
Lukman Oktadianto
1
;
Mia Ratwita Andarsini
1
;
I Dewa Gede Ugrasena
1
;
Yetti Hernaningsih
2
;
Andi Cahyadi
1
;
Maria Christina Shanty Larasati
1
Author Information
1. Hematology-Oncology Division, Department of Pediatrics, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Hospital, Surabaya 60285, Indonesia
2. Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga/Dr.Soetomo General Academic Hospital, Surabaya 60285, Indonesia
- Publication Type:Journal Article
- Keywords:
Acute lymphoblastic leukemia, Cancer, Caspase-3, Childhood leukemia, Chemotherapy
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.3):91-97
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Caspase-3 is a crucial mediator of the extrinsic apoptosis pathway. The role of caspase-3 for extrinsic
apoptosis signalling is still a challenge and should be exploited in childhood ALL. This study aimed to compare
the caspase-3 expression in the patient’s bone marrow before and after the induction phase of chemotherapy in
childhood ALL. It will also to correlate the mean difference in caspase-3 expression between ALL standard-risk and
ALL high-risk patients. Methods: Seventeen newly diagnosed ALL subjects were enrolled in this study. Caspase-3
expression in bone marrow was assessed using flow cytometry and monoclonal antibodies. A T-test and a paired
T-test were used to compare between groups. The correlation coefficient between ALL groups was evaluated using
Spearman’s test and linear regression with a significant p-value of 0.05. Results: The caspase-3 expression is higher
after induction therapy. However, it showed an insignificant difference (16.56+12.91% vs 27.71+12.33%; p = 0.08,
p > 0.05). The mean difference of caspase-3 in ALL high-risk groups was significantly higher than in ALL standard-risk
groups with a positive correlation (p = 0.007, r = 0.756). Conclusion: The caspase-3 expression after induction phase
chemotherapy was increased in all standard-risk and high-risk patients; other lymphoblast apoptosis markers need to
be confirmed alongside caspase-3.
- Full text:11.2023my1512.pdf
