Antibacterial and Wound Healing Activity of 2% Formulation of 2-Medpy-3-CN on Infected Burn Wounded Animal Model
https://doi.org/10.47836/mjmhs.19.3.2
- Author:
Vasantha Kumari Neela
1
;
Nor Maziah Ahmad
1
;
Surajo Adamu Wada
1
,
2
;
Arun Kumar Karunanidhi
1
Author Information
1. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia&
2. Department of Microbiology Faculty of Pure and Life Sciences, Federal University, Wukari, 200 Katsina-Ala Road Wukari, Taraba State Nigeria
- Publication Type:Journal Article
- Keywords:
Allium stipitatum, Burn wound, Antibacterial, MRSA, Silver sulfadiazine
- From:Malaysian Journal of Medicine and Health Sciences
2023;19(No.3):5-12
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Humans have learned to recognize and process plants into medicinal forms through centuries. Burns
can spread to other tissues, especially when infected with bacteria such as Methicillin-Resistant Staphylococcus
aureus (MRSA). The study aimed to assess the in vivo antibacterial and wound healing activity of 2% formulation
of 2-Medpy-3-CN on infected burn wounded animal model. Methods: In vitro antibacterial activity of the Alsti was
done by broth dilution and disc diffusion methods. Alsti 2% ointment was prepared for the infected burn wound
treatment. A total of 18 rats are grouped into A, B, C, and D, the first three groups (A-C) were injured thermally, and
Group D was used as healthy controls. The three test Groups were exposed to MRSA ATCC 43300 at 105 CFU/mL.
Group A was treated with 2% Alsti, Group B with Silver sulfadiazine 1% (SSD), and Group C was untreated. Wounds
healing was assessed by the healed area and microscopic identification of hematoxylin and eosin (H&E)-stained skin
tissue. Results: Wound healing progresses with application of Alsti 2% ointment as observed through wound diameter and histopathological changes of the skin. Wound diameter decreases with treatments, while the contrary was
observed in the non-treated group. Microscopic observation of the stained skin showed that epidermal development,
and collagen formation progress with treatment days. Untreated wounds showed marked inflammation, progressive ulceration, and necrosis. Conclusion: Alsti 2% formulation showed antibacterial and wound healing activities,
hence, can be used as alternative in burn wound infections.
- Full text:11.2023my1502.pdf
