Construction of albumin nanoparticles loading PROTAC and its inhibition effect on NAD+ in glioma cells
10.12206/j.issn.2097-2024.202307027
- VernacularTitle:载PROTAC分子白蛋白纳米粒的构建及对胶质瘤细胞NAD+代谢的抑制
- Author:
Hongbo WANG
1
;
Lingyi GUO
1
;
Wenya CHI
1
;
Kangqing BIAN
1
;
Wenbo ZHOU
1
;
Yuan YU
2
,
3
Author Information
1. Department of Pharmaceutical Science, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. Department of Pharmaceutical Science, School of Pharmacy, Naval Medical University, Shanghai 200433, China
3. Key Laboratory of Biosafety Defense(Naval Medical University) , Ministry of Education, Shanghai 200433, China.
- Keywords:
Glioma;
Nanoparticle;
Albumin;
PROTAC;
HPLC
- From:
Journal of Pharmaceutical Practice
2023;41(10):594-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and optimize the formulation of Albumin nanoparticles loading PROTAC molecule and observe the inhibition effect of nanoparticles on the proliferation and NAD+ metabolism of glioma cells. Methods Albumin nanoparticles loading NPT-B2 were prepared and characterized with a thermal driving method, and the prescription was optimized. An HPLC method was established to determine the content of NPT-B2. The proliferation inhibition of NPT-B2 and B2-BSA-NPs on U251 cells were investigated by the CCK8 method, and the degradation effects of NPT-B2 and B2-BSA-NPs on NAMPT in glioma cells were investigated by western blotting. Results The HPLC method was stable, with good linearity, precision, and recovery rate. The nanoparticles had a particle size of about 55.48 nm, a potential of about −12.9 mV, an encapsulation rate of about 94.74%, and a drug loading amount of about 8.61%. The IC50 of NPT-B2 on glioma cells was 61.16 nmol/L, which had a degradation effect on NAMPT. The IC50 of B2-BSA-NPs on glioma was 41.21 nmol/L, which had a very significant degradation effect on NAMPT. Conclusion Albumin nanoparticles loading PROTAC molecules were constructed. The prescription was optimized to improve the drug encapsulation rate, and the low water solubility of PROTAC molecule was improved, which had a significant inhibitory effect on the proliferation and NAD+ energy metabolism of glioma cells.
