Quality Evaluation of Aurantii Fructus from Sichuan by Specific Chromatogram Combined with Chemometrics and Grey Correlation Analysis
10.13422/j.cnki.syfjx.20231068
- VernacularTitle:特征图谱结合化学计量学与灰色关联度的川产枳壳药材质量评价
- Author:
Chunying RAN
1
;
Jie WANG
1
;
Yingxin CHEN
1
;
Yanhong MA
1
;
Lili YIN
1
;
Haohan WANG
1
;
Xiaoyang CAI
1
;
Zhi LI
1
;
Min LI
1
;
Tianzhi ZHANG
2
Author Information
1. State Key Laboratory of Southwestern Chinese Medicine Resources，School of Pharmacy/School of Modern Chinese Medicine Industry，Chengdu University of Traditional Chinese Medicine， Chengdu 611137，China
2. Sichuan Tianzhi Chinese Herbal Medicine Co. Ltd.，Luzhou 646001，China
- Publication Type:Journal Article
- Keywords:
Aurantii Fructus;
high performance liquid chromatography（HPLC）;
specific chromatogram;
cluster analysis;
principal component analysis（PCA）;
grey correlation analysis;
chemometrics;
entropy weight method
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(22):121-127
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveHigh performance liquid chromatography （HPLC） was used to establish the specific chromatograms of Aurantii Fructus from different origins， and the quality variability of Aurantii Fructus from Sichuan was analyzed and evaluated by combining entropy weighting method and grey correlation method. MethodHPLC was performed on an Agilent Eclipse Plus C18 column （4.6 mm×250 mm， 5 μm） with a gradient elution of methanol （A）-0.1% phosphoric acid aqueous solution as the mobile phase （0-12 min， 25%-33%A； 12-21 min， 33%-41%A； 21-30 min， 41%-42%A； 30-40 min， 42%-59%A； 40-53 min， 59%-72%A； 53-60 min， 72%A； 60-65 min， 72%-100%A； 65-70 min， 100%A； 70~71 min， 100%-25%A； 71-80 min， 25% A） at a flow rate of 1.0 mL·min-1， the injection volume was 10 μL and the detection wavelength was 330 nm. Fifty batches of Aurantii Fructus samples from different origins （Sichuan， Chongqing， Jiangxi and Hunan） were tested， and the similarity evaluation software is used to generate characteristic profiles and compare them with control profile for peak identification， and then to evaluate the similarity of the samples. IBM SPSS 19.0 and SIMCA 14.1 were used to perform multivariate statistical analysis on the results of the samples， and then the entropy weighting method and grey correlation were used to calculate the overall quality score of samples from Sichuan. ResultHPLC specific chromatogram of Aurantii Fructus was established， and 14 common peaks were identified as eriocitrin， neoeriocitrin， narirutin， naringin， hesperidin， neohesperidin， meranzin hydrate， poncirin， meranzin， marmin， nobiletin， 3，3′，4′，5，6，7，8-heptamethoxyflavone， tangeretin and auraptene. And the similarities between the samples from Sichuan and the control chromatogram were all above 0.980. The samples could be classified into four categories according to their main origins by chemical pattern recognition， and the results of cluster analysis， principal component analysis and orthogonal partial least squares-discriminant analysis were all able to discriminate the samples of different main origins effectively. The comprehensive evaluation results of entropy weighting method combined with grey correlation showed that the quality of Aurantii Fructus from Sichuan varied greatly among different origins， and the quality of Aurantii Fructus from Sichuan was ranked as Bazhong>Luzhou>Chongqing>Neijiang. ConclusionIn this study， the characteristic mapping of Aurantii Fructus from Sichuan is established， and combined with the analytical methods of chemometrics and grey correlation， the quality of samples from different origins can be effectively differentiated， which can provide a reference for the comprehensive evaluation and control of the quality of Aurantii Fructus from Sichuan.
