Study on Mechanism of Action of Matrine in Treatment of Inflammatory Bowel Disease Based on Zebrafish Model and Network Pharmacology
10.13422/j.cnki.syfjx.20230914
- VernacularTitle:基于斑马鱼模型与网络药理学研究苦参碱治疗炎症性肠病的作用机制
- Author:
Linzhen CHEN
1
;
Xue ZHANG
1
;
Qi CHEN
1
;
Xue YU
2
;
Shengyun DAI
3
;
Zhiqiang MA
1
;
Chongjun ZHAO
1
Author Information
1. Beijing Key Laboratory of Traditional Chinese Medicine（TCM） Quality Evaluation， Beijing University of Chinese Medicine， Beijing 102488， China
2. School of TCM， Beijing University of Chinese Medicine， Beijing 100029， China
3. National Institutes for Food and Drug Control， Beijing 102629， China
- Publication Type:Journal Article
- Keywords:
matrine;
inflammatory bowel disease;
zebrafish;
network pharmacology;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(22):88-94
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the mechanism of matrine in the treatment of inflammatory bowel disease （IBD） based on the zebrafish model and network pharmacology. MethodThe IBD model of zebrafish was established using 2，4，6-trinitro-benzenesulfonicacid （TNBS）， and the intestinal phagocytic function， goblet cell secretion， and neutrophil aggregation were evaluated using neutral red staining， alcian blue staining， and neutrophil number changes. Changes in tumor necrosis factor （TNF）-α and cholecystokinin （CCK） content in zebrafish were determined by using relevant reagent kits. Network pharmacology and molecular docking techniques were used to predict the potential mechanism of matrine in the treatment of IBD. Gene expression of relevant targets was verified through Real-time polymerase chain reaction （Real-time PCR）. ResultCompared with the model group， the matrine administration group can increase the neutral red staining area in a dose-dependent manner and improve intestinal phagocytic function（P<0.05，P<0.01）. It can reduce the staining area of alcian blue and affect the secretion of intestinal goblet cells（P<0.01）. It can reduce the number of neutrophil granulocytes， relieve its aggregation， significantly reduce TNF-α content（P<0.01）， and increase the CCK content. Network pharmacology analysis identifies 28 potential targets for matrine in the treatment of IBD. The top five targets by protein-protein interaction （PPI） network analysis are CHRNA7， DRD1， CHRNA4， SLC6A3， and GRM5. The Kyoto encyclopedia of genes and genomes （KEGG） results show that the treatment of IBD with matrine may be related to neuroactive ligand-receptor interaction， cholinergic synapse， and neutrophil extracellular trap formation. Real-time PCR results show that matrine can affect the expression level of related target genes. Conclusionmatrine has a certain therapeutic effect on IBD and can affect the inflammatory response of IBD. Its therapeutic effect may be related to neuroactive ligand-receptor interaction and other pathways.
