Anticancer effect of therapy by antibiotic combined with programmed cell death protein 1 in H_(22) hepatocellular carcinoma mouse model
10.13200/j.cnki.cjb.003736
- VernacularTitle:抗生素联合抗程序性细胞死亡蛋白1抗体治疗在H_(22)肝癌小鼠模型中的抗肿瘤作用
- Author:
LIU Li
- Publication Type:Journal Article
- Keywords:
Antibiotics(ATB);
Immunotherapy;
Programmed cell death protein 1 antibody;
Hepatocellular carcinoma
- From:
Chinese Journal of Biologicals
2023;36(10):1185-1191+1197
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the influence of immunotherapy by antibiotic(ATB) combined with programmed cell death protein 1(PD1) on curative effect of hepatocellular carcinoma in mice.Methods H_(22) tumor-bearing male BALB/c mouse model was established.Eight model mice were injected i.p.with anti-PD1,250 μg for each,and administered intragastrically with ceftriaxone sodium(100 mg/kg) plus lincomycin hydrochloride(200 mg/kg),using eight normal mice administered intragastrically with normal saline(0.2 mL for each) as control.The anticancer effect of ATB combined with PD1 was evaluated by tumor size,hematoxylin-eo sin(HE) staining of tumor tissue and spleen index.The secretion levels of IL-2,IL-10 and IFN_γ in sera of mice were determined by ELISA,while the proportions of CD3~+ and CD8~+ T cells in mouse lymphocytes by flow cytometry,and the expression levels of CD3 and CD8 in mouse tumor tissue by immunohistochemistry(IHC) method.Results The therapy by ATB combined with anti-PD 1 showed significantly inhibitory effect on tumor growth,which increased the proportions of CD3~+ and CD8~+ T cells in T lymphocytes,up-regulated the secretion levels of IL-2,IL-10 and IFN_γ,and regulated the immune function of T cells by up-regulating the expression levels of CD3 and CD8 in tumor tissue to continuously activate the immune system and suppress the tumor.Conclusion Narrow-spectrum ATB may promote the effect of anti-PD 1 immunotherapy on tumors by improving immune function.
