Screening of solid culture medium without animal-derived materials for resuscitation of Streptococcus pneumoniae
10.13200/j.cnki.cjb.004011
- VernacularTitle:肺炎球菌菌种复苏用无动物源性固体培养基的筛选
- Author:
WANG Yanyan
- Publication Type:Journal Article
- Keywords:
Streptococcus pneumoniae(Spn);
Culture medium without animal-derived materials;
Solid culture medium;
Hereditary stability;
Suitability for production
- From:
Chinese Journal of Biologicals
2023;36(10):1172-1178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen a kind of solid culture medium without animal-derived materials for resuscitating Streptococcus pneumoniae(Spn).Methods Spn 19F was selected for the preliminary screening of 9 kinds of formula culture media without animal-derived materials,and then other 23 serotypes of Spn were cultured for verification.The effect of the selected medium on the hereditary stability of various serotypes was identified by strain identification and capsular polysaccharide antigen gene sequence analysis of the 15-generation strains.The strains resuscitated by this medium were fermented according to the production procedure,and the culture situation and the yield and quality of capsular polysaccharide antigen were analyzed to determine the production applicability of the screened medium.Results The number of viable bacteria of various types of Spn was desired after 11～15 h of incubation on the selected solid medium and there was no difference in strain identification and antigen gene sequence between the final 15 generation and the control(strains cultured in sheep blood solid medium).After fermentation and culture of the strain resuscitated by this medium,the cell growth and the capsular polysaccharide production increased slightly,and all the related verification indicators of capsular polysaccharide met the requirements in Chinese Pharmacopoeia(Volume Ⅲ,2020 edition).Conclusion The selected solid culture medium without animal-derived materials has good applicability and can replace blood-derived culture medium for the resuscitation of Spn strain for vaccine production.
