Expression of PLCH1 gene in esophageal squamous cell carcinoma and its effect on cell proliferation and migration
10.13200/j.cnki.cjb.003994
- VernacularTitle:PLCH1基因在食管鳞状细胞癌中的表达及其对细胞增殖、迁移的影响
- Author:
BIAN Lin
- Publication Type:Journal Article
- Keywords:
PLCH1gene;
Esophageal squamous cell carcinoma(ESCC);
Copy number amplification;
Oncogene
- From:
Chinese Journal of Biologicals
2023;36(9):1097-1104
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect the gene variation and expression of PLCH1 in esophageal squamous cell carcinoma(ESCC),analyze the function of PLCH1 gene in ESCC and explore its mechanism.MethodsThe copy number variation of PLCH1 in ESCC was analyzed by GISTIC,and the expression of PLCH1 in ESCC and normal esophageal tissues was detected by TCGA database and immunohistochemistry method. The expression of PLCH1 in ESCC cell lines was detected by real-time fluorescence quantitative PCR(qPCR) and Western blot,and the effects of PLCH1 silencing on the proliferation and migration of ESCC cells were detected by MTT assay,colony formation assay and Transwell assay.Results There was significant copy number amplification of PLCH1 in ESCC(G-scores > 0. 1,P < 0. 05),and the expression levels of PLCH1 mRNA and protein in ESCC were significantly higher than those in normal tissues(F = 36. 00 ～ 1 101. 00respectively,each P < 0. 000 1). After PLCH1 silencing,the ability of proliferation,clone formation and migration of ESCC cells KYESE180 and TE-9 decreased significantly(F = 35. 49 ～ 634. 00 respectively,each P < 0. 001).Conclusion PLCH1 plays an oncogenic role in ESCC,which is of great significance for the metastasis and proliferation of ESCC,and can be used as a potential target for the treatment of ESCC.
