Progress in research on detection techniques for peste des petits ruminants virus
- VernacularTitle:小反刍兽疫病毒检测技术的研究进展
- Author:
HE Yunjiang
- Publication Type:Journal Article
- Keywords:
Peste des petits ruminants virus(PPRV);
Detection techniques;
Molecular biology
- From:
Chinese Journal of Biologicals
2023;36(8):1014-1020
- CountryChina
- Language:Chinese
-
Abstract:
Peste des petits ruminants(PPR)is an acute and highly contagious disease caused by peste des petits ruminants virus(PPRV),which mainly infects goats and sheep with high morbidity and mortality. Because of its serious pathological damage and wide spread,PPR has caused huge economic losses to the aquaculture industry and international trade in various countries,so it is particularly important to establish a rapid and accurate detection method for the prevention and control of the disease. This paper reviews the progress in research on biological detection techniques for PPRV detection,such as routine RT-PCR,routine multiplex PCR,real-time fluorence quantitative PCR(RT-qPCR),pyrosequencing,nested PCR(nPCR),loop-mediated isothermal amplification(LAMP)and recombinase polymerase amplification(RPA),so as to provide bases and ideas for scientific detection and identification of PPRV.
