Application progress of high performance liquid chromatography in detection of hemagglutinin content in influenza vaccine

VernacularTitle:高效液相色谱在流感疫苗血凝素含量检测中的应用进展
Author: TONG Guangwei
Publication Type:Journal Article
Keywords: Influenza vaccine; Chromatographic column; Hemagglutinin(HA); Single-radial immunodiffusion(SRID); Reversed phase-high performance liquid chromatography(RP-HPLC); Size exclusion-high performance liquid chromatography(SE-HPLC)
From: Chinese Journal of Biologicals 2023;36(8):1005-1009
CountryChina
Language:Chinese
Abstract: At present,the most commonly used method for detecting hemagglutinin(HA)content in influenza vaccines is still single-radial immunodiffusion(SRID). However,the preparation of standards required by this method takes a long time,usually 2 ～ 3 months. Therefore,how to quantitatively analyze HA accurately has always been a difficult problem in the detection of HA content in the situation that reference products can not be obtained at the early stage of the pandemic influenza. High performance liquid chromatography(HPLC)has its own characteristics of rapidity,high sensitivity,good repeatability and high accuracy,which can rapidly determine HA content by using different separation principles and has been widely used in the detection of HA content in influenza vaccine. This paper reviewed the research progress of the application of HPLC in the determination of HA content in influenza vaccine.