Metabolomics study on occupational acute methyl acetate poisoning using patient plasma
10.20001/j.issn.2095-2619.20230402
- VernacularTitle:职业性急性乙酸甲酯中毒患者血浆代谢组学研究
- Author:
Yiru QIN
1
;
Anping MA
;
Jingjing QIU
;
Yin HAN
;
Jiayun WU
;
Zuofei XIE
;
Yangle SUN
;
Junyi HUANG
;
Ming DONG
;
Weifeng RONG
;
Banghua WU
;
Shijie HU
Author Information
1. Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong 510300, China
- Publication Type:Journal Article
- Keywords:
Methyl acetate;
Acute poisoning;
Plasma;
Metabolomics;
Lipids;
Folate;
Amino acids;
Metabolic pathway
- From:
China Occupational Medicine
2023;50(2):127-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the specificity of endogenous metabolic profile in plasma of patients with occupational acute methyl acetate poisoning using non-targeted metabolomics. Methods: A total of six patients with occupational acute methyl acetate poisoning were selected as the poisoning group, while 10 healthy workers without occupational exposure history of chemical hazards in the same industry were selected as the control group using the judgment sampling method. Metabolites in patient plasma of the two groups were detected using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, and non-targeted metabolomics analysis was performed. Principal component analysis and partial least squares discriminant analysis were used to identify differential metabolites and analyze their metabolic pathways. Results: There were significant differences in metabolite profiles in patient plasma between poisoning group and control group. A total of 195 differentially expressed metabolites were screened in plasma of patients in poisoning group, including 119 upregulated and 76 downregulated metabolites. Lipid substances (lipids and lipid-like molecules) accounted for the highest proportion (21.5%). The differential metabolites of poisoning group were related to folate biosynthesis, amino acid metabolism, pyrimidine metabolism, sphingolipid biosynthesis and other metabolic pathways in plasma compared with the control group (all P<0.05). Conclusion: Occupational acute methyl acetate poisoning affects metabolism of the body. The folic acid biosynthesis, amino acid and lipid metabolism and other pathways may be involved in the occurrence and development of poisoning.
