Study on Changes of Microorganisms and Volatile Components in Aquilariae Lignum Resinatum with Different Aging Years
10.13422/j.cnki.syfjx.20231113
- VernacularTitle:不同陈化年份沉香中微生物及挥发性成分的变化
- Author:
Linfan ZHANG
1
;
Jiaqi GAO
2
;
Anjun WANG
3
;
Tianxiao LI
1
;
Juan LIU
1
Author Information
1. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
2. Institute of Traditional Chinese Medicine（TCM）， Tianjin University of TCM， Tianjin 301617， China
3. School of Pharmacy， Jiangsu University， Zhenjiang 212013， China
- Publication Type:Journal Article
- Keywords:
Aquilaria sinensis;
Aquilariae Lignum Resinatum;
aging;
microbial diversity;
volatile components
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(21):155-164
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis study aims to investigate the changes in fungal community diversity and volatile components during the aging process of Aquilariae Lignum Resinatum and explore the internal relationship between them. MethodAquilariae Lignum Resinatum samples with different aging years were collected. High-throughput sequencing was employed to analyze the fungal diversity and abundance， and α and β diversity indicators were calculated to reveal the composition and dynamic changes of the fungal community. In addition， the essential oils of Aquilariae Lignum Resinatum with different aging years were extracted， separated， and identified by two-dimensional gas chromatography-high resolution time-of-flight mass spectrometry. ResultA total of 61 compounds were identified from the volatile components of five groups of Aquilariae Lignum Resinatum samples， including 2 monoterpenes， 24 sesquiterpenoids， 1 diterpene， 13 aromatic hydrocarbons， 9 alkanes， and 12 other compounds. Among them， the volatile compounds isolated from the sample aged for 1 year had the largest number， and those from the sample aged for 2 years accounted for the largest proportion of the total components. The internal transcribed spacer（ITS） amplicon sequencing revealed that the fungi in the five groups of samples belonged to 162 genera. Kirschsteiniothelia， Aspergillus， Lasiodiplodia， Phaeoacremonium， and Trichoderma were the dominant fungal genera. The fungal diversity in the Aquilariae Lignum Resinatum sample aged for 4 years was significantly higher than that in the samples aged for 0 to 3 years. ConclusionThe volatile component content and composition of Aquilariae Lignum Resinatum altered dramatically during aging. The aging of Aquilariae Lignum Resinatum was accompanied by the increasing fungal diversity， decreasing relative content of aromatic hydrocarbons， and increasing relative content of sesquiterpenoids. In general， aging was beneficial to the transformation of sesquiterpenoids and the enrichment of fungi.
