Effect of long non-coding RNA MALAT1 on growth and invasion of thyroid cancer cells and its mechanism

WANG Jinlin

白话标题:长链非编码RNA MALAT1对甲状腺癌细胞生长和侵袭的影响及其作用机制
作者: WANG Jinlin
出版类型:Journal Article
来源: Chinese Journal of Biologicals 2023;36(7):786-792
国家China
语言:Chinese
摘要: Objective To evaluate the effect of long non-coding RNA(lncRNA) MALAT1 on the growth and invasion of thyroid cancer cells and explore its mechanism.Methods The relative expression levels of MALAT1 and miR-211-5p genes were detected by real-time quantitative PCR in cancer tissues and adjacent tissues of 44 patients with thyroid cancer,and the relative expression levels of MALAT1 gene in SW1736,TC1,Hth7 and Nthy-ori 3-1 cells were detected by the same method.MTT assay was used to detect the effect of MALAT1 on the survival of thyroid cancer cells(SW1736 cells were transfected with pcDNA3.1-MALAT1,pcDNA3.1,si-MALAT1 and si-NC,respectively),using which the effect of overexpression of miR-211-5p on the survival of thyroid cancer cells induced by MALAT1(SW1736 cells were transfected with pcDNA3.1-MALAT1,miR-211-5p mimics,Scramble and pcDNA3.1-MALAT1+miR-211-5p mimics,respectively) was detected.Transwell chamber assay was used to detect the effect of MALAT1 on the invasive ability of thyroid cancer cells(SW1736 cells were transfected with si-NC,si-MALAT1, pcDNA3.1,pcDNA3.1-MALA T1, miR-211-5pmimics and miR-211-5p mimics+pcDNA-MALAT1,respectively).The regulation of miR-211-3p expression by MALAT1 was detected by real-time quantitative PCR(SW1736 cells were transfected with pcDNA3.1-MALAT1,pcDNA3.1,si-MALAT1 and si-NC,respectively).Results In 44 thyroid cancer tissue samples,compared to adjacent tissues,MALAT1 gene was highly expressed in37 samples(84.1%),while miR-211-5p was down-regulated in 39 samples(88.6%).The expression of MALA T1 was negatively correlated with that of miR-211-5p in thyroid cancer samples(r~2=0.168,P=0.005 7).Compared with Nthy-ori 3-1 cells,the relative expression level of MALAT1 gene in SW1736,TC1 and Hth7 cells increased significantly(t=10.230,5.813and 74.310,P=0.009,0.004 and <0.001,respectively).At 72 h after transfection,the growth ability of SW1736 cells in si-MALAT1 group decreased significantly(t=7.780,P=0.001),while that in pcDNA3.1-MALAT1 group increased significantly(t=-7.410,P=0.002) compared with that in si-NC group;Compared with pcDNA3.1-MALAT1 group,the growth ability of SW1736 cells in pcDNA3.1-MALAT1+miR-211-5p mimics group decreased significantly(t=-5.640,P=0.005).Compared with si-NC group,the number of invasive SW1736 cells in si-MALAT1 and miR-211-5p mimics group decreased significantly(t=-6.710 and-6.290,respectively,each P=0.003),while increased significantly in pcDNA3.1-MALAT1 group(t=5.740,P=0.005).Compared with pcDNA3.1 group,the relative expression level of miR-211-5p gene in pcDNA3.1-MALAT1 group decreased significantly(t=-7.510,P=0.002);Compared with si-NC group,miR-211-5p gene expression in si-MALAT1 group increased significantly(t=7.960,P=0.001).Conclusion Interfering the expression of lncRNA MALAT1 can effectively inhibit the proliferation and migration of thyroid cancer cells,which might be achieved by up-regulating the expression level of miR-211-5p.MALAT1 is expected to be a potential molecular target for the treatment of thyroid cancer.