Tangeretin inhibits tumor stemness of non-small cell lung cancer by regulating PI3K/AKT/mTOR signaling pathway
- VernacularTitle:桔皮素通过调节PI3K/AKT/mTOR信号通路抑制非小细胞肺癌的肿瘤干性
- Author:
Sai WANG
1
;
Lingjie WANG
1
;
Yanli LI
1
;
Peng LI
1
;
Mengjun LI
1
;
Donghua ZHAO
1
;
Yongjie WANG
2
Author Information
1. Qingdao University, Qingdao, 266071, Shandong, P. R. China
2. Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266071, Shandong, P. R. China
- Publication Type:Journal Article
- Keywords:
Tangeretin;
non-small cell lung cancer;
cancer stemness;
PI3K/AKT/mTOR signaling pathway Foundation item: Young Scientists Fund of the National Natural Science Foundation of China (81601602)
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2023;30(04):614-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Tangeretin on non-small cell lung cancer (NSCLC) and the tumor stemness, and to find the molecular mechanism of its effect. Methods We used cell counting and cell cloning experiments to study the effect of Tangeretin on the proliferation of NSCLC cells in vitro. The effect of Tangeretin on the invasion of NSCLC cells was detected by transwell assay. We detected the effect of Tangeretin on the proliferation of NSCLC cells in vivo by nude mouse tumor-bearing experiment. The effect of Tangeretin on tumor stemness of NSCLC cells was detected by self-renew assay, and CD133 and Nanog protein expressions. The expressions of PI3K/AKT/mTOR signaling pathway-related proteins were detected by Western blotting (WB). Results Tangeretin had a good inhibitory effect on the proliferation of NSCLC cells in vivo and in vitro. Cell counting experiment, clonal formation experiment and nude mouse tumor-bearing experiment showed that Tangeretin could inhibit the proliferation activity, clonal formation ability, and tumor size of NSCLC cells in vivo. Self-renew experiments showed that Tangeretin could inhibit the self-renew ability of NSCLC cells. WB experiments showed that Tangeretin inhibited the expressions of tumor stemness markers CD133 and Nanog in NSCLC cells. Tangeretin could inhibit the activation of PI3K/AKT/mTOR signaling pathway-related proteins in NSCLC cells, and the activation of PI3K/AKT/mTOR signaling pathway could partially remit the inhibitory effect of Tangeretin on tumor stemness of NSCLC cells. Conclusion Tangeretin can inhibit the tumor stemness of NSCLC cells, which may be related to the regulation of PI3K/AKT/mTOR signaling pathway.
