Optimization of culture conditions of SARS-CoV-2 four Omicron variants in Vero cells
10.13200/j.cnki.cjb.003925
- VernacularTitle:SARS-CoV-2 4种奥密克戎变异株在Vero细胞中培养条件的优化
- Author:
XIA Fei
- Publication Type:Journal Article
- Keywords:
Severe acute respiratory symptom coronavirus 2(SARS-CoV-2);
Omicron variants;
Vero cells;
Culture conditions;
Vaccine
- From:
Chinese Journal of Biologicals
2023;36(6):641-646
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the culture conditions of four vaccine candidates of severe acute respiratory symptom coronavirus 2(SARS-CoV-2) Omicron variants BA.1,BA.1.1,BA.2 and BA.5 in Vero cells.Methods The harvest time(24,48,72 and 96 h) and MOI(0.01,0.001,0.0001 and 0.000 01) of four Omicron variants cultured in Vero cells were optimized by using cytopathic effect(CPE),viral nucleic acid copy number and viral titer as evaluation indexes.Results The optimum harvest time of the four Omicron variants BA.1,BA.1.1,BA.2 and BA.5 in Vero cells was 72 h,and the optimum MOI was 0.001～0.000 01,0.001～0.000 01,0.01～0.000 01 and 0.01～0.000 01,respectively.Conclusion The culture conditions of four Omicron variants in Vero cells were optimized,which laid a foundation of the development of SARS-CoV-2 Omicron variant inactivated vaccine based on Vero cells.
