Bioinformatics analysis of VP1 of Coxsackievirus A10
10.3969/j.issn.1006-2483.2023.05.003
- VernacularTitle:柯萨奇病毒A组10型VP1的生物信息学分析
- Author:
Gaobo ZHANG
1
;
Yiwen LIU
2
;
Zixing LI
2
;
Yiyang LIU
2
;
Juan YUAN
3
,
4
Author Information
1. Hubei Gedian Humanwell Pharmaceutical Excipients Co.,Ltd. Ezhou , Hubei 436070, China
2. Wuhan Huaxia Institute of Technology , Wuhan , Hubei 430223 , China
3. Wuhan Dongxihu District People'
4. s Hospital , Wuhan , Hubei 430040 , China
- Publication Type:Journal Article
- Keywords:
Hand, foot, and mouth disease (HFMD);
Coxsackievirus A10 (CV-A10);
Viral protein 1 (VP1);
Bioinformatics;
Antigenic epitope
- From:
Journal of Public Health and Preventive Medicine
2023;34(5):14-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To predict and analyze the physicochemical properties, structural characteristics, and antigenic epitopes of viral protein (VP) VP1 of Coxsackievirus A10 (CV-A10) by bioinformatics methods. Methods The physicochemical properties and structural characteristics of CV-A10 VP1 were predicted by ProtParam, SOPMA, SWISS-MODEL, PDBsum, and ProSA-web. The antigenic epitopes of CV-A10 VP1 were predicted and analyzed by DNAstar, ABCpred, Bepipred 2.0, ElliPro, DiscoTope-2.0, NetMHCpan-4.1, NetMHCIIpan-4.0, Consurf, VaxiJen v.2.0, AllerTOP v.2.0, ToxinPred2, and IEDB immunogenicity. Results Bioinformatics analysis showed that CV-A10 VP1 was a basic, unstable, and hydrophilic protein, of which the secondary structure mainly consisted of random coil. The analysis revealed that CV-A10 VP1 had multiple potential B and T cell antigenic epitopes as well as a dominant antigenic epitope based on the potential epitope. Conclusion CV-A10 VP1 has multiple potential sites that induce specific humoral and cellular immunity, providing important support for its experimental identification, molecular epidemiological studies, and vaccine development.
