Mechanism study of Qingre huashi decoction in the treatment of gastric cancer by up-regulating miRNA-155 and inhibiting Wnt/β-catenin signaling pathway
- VernacularTitle:清热化湿方通过上调miRNA-155抑制Wnt/β-catenin信号通路治疗胃癌的作用机制研究
- Author:
Shuoxin YIN
1
;
Xianwei AO
2
;
Bo LI
3
;
Tao ZHANG
4
;
Yuanneng CHEN
4
Author Information
1. Nanyang Central Hospital，Henan Nanyang 473000，China
2. Dept. of Gastroenterology，the Second People’s Hospital of Jinzhou，Hubei Jinzhou 434000，China
3. Dept. of Respiratory Medicine，Nanyang Central Hospital，Henan Nanyang 473000，China
4. Dept. of Gastroenterology，Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine，Nanning 530011，China
- Publication Type:Journal Article
- Keywords:
Qingre huashi decoction;
miRNA-155;
gastric
- From:
China Pharmacy
2023;34(19):2339-2343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the mechanism of Qingre huashi decoction in the treatment of gastric cancer by intervening in miRNA-155 and inhibiting Wnt/β-catenin signaling pathway. METHODS Thirty nude mice were randomly divided into model group， control group （0.004 g/kg cisplatin+0.02 g/kg fluorouracil）， overexpression group， Qingre huashi prescription low-dose， medium-dose and high-dose groups （2.71， 5.43， 10.86 g/kg）， with 5 mice in each group. The overexpression group was inoculated with miRNA-155 AGS cell line， and the other groups were inoculated with AGS cells to induce tumor-bearing gastric cancer model. The control group was given relevant medicine intraperitoneally， and other groups were given relevant medicine or normal saline intragastrically， once a day， for 3 consecutive weeks. The weight of tumor tissue in nude mice was determined； the pathological morphology of tumor tissue was observed； the miRNA-155 expression， mRNA and protein expressions of Wnt7， β-catenin and T- cell factor-4（TCF-4） in tumor tissue were detected. RESULTS Compared with the model group， the tumor weights of nude mice in the control group， the overexpression group and Qingre huashi decoction high-dose group were significantly reduced （P＜0.05）； mRNA and protein expressions of Wnt7， β -catenin and TCF-4 were significantly decreased （P＜0.05）， while miRNA-155 expression was increased significantly （P＜0.05）. Tumor cells exhibited varying degrees of loose arrangement， shallow nuclear staining， and necrotic foci. CONCLUSIONS Qingre huashi decoction can inhibit the protein and mRNA expressions of Wnt7， β-catenin and TCF-4 in Wnt/β-catenin signaling pathway by up-regulating miRNA-155， thus inhibiting the tumor growth of tumor-bearing nude mice.
