Identification of components，fingerprint and content determination of the effective parts of Dracocephalum moldavica
- VernacularTitle:香青兰有效部位的成分鉴定、指纹图谱建立及含量测定
- Author:
Chenghui HE
1
,
2
,
3
;
Honghong LI
2
;
Jiamin LYU
2
;
Guoan ZOU
1
;
Asia HAJIAKBER
1
Author Information
1. State Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization，Xinjiang Technical Institute of Physics and Chemistry，Chinese Academy of Sciences，Urumqi 830011，China
2. Xinjiang Medicine Research Institute，Urumqi 830004，China
3. University of Chinese Academy of Sciences，Beijing 100049，China
- Publication Type:Journal Article
- Keywords:
Dracocephalum moldavica;
effective parts;
component identification;
fingerprint;
content determination;
quality
- From:
China Pharmacy
2023;34(19):2328-2332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide a reference for comprehensive quality evaluation and control of the effective parts of Dracocephalum moldavica （EPDM）. METHODS A total of 10 batches of EPDM were prepared， and chemical information of EPDM was collected by HPLC-Q-Exactive-MS. EPDM components were identified by literature search， database comparison and manual analysis. HPLC fingerprints of 10 batches of EPDM were established by using the Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprint （2004 A edition）； the similarity evaluation and common peak identification were carried out， and the contents of 5 index components were determined by HPLC. RESULTS A total of 11 compounds in EPDM were identified. The fingerprint similarities of EPDM samples from 10 batches were all above 0.96. Among 11 chromatographic peaks， 5 peaks were identified， such as luteolin-7-O-β-D-glucuronide（LG）， apigenin-7-O-glucuronide（APG）， rosmarinic acid（RA）， diosmetin-7-O-β-D-glucuronide（DG）， tilianin（TL） . The results of the quantitative analysis showed that all above 5 components had good linearity （R2≥0.999）， and the average recoveries were in the range of 95.12%-98.37%. The contents of LG， APG， RA， DG， TL were 21.268 3-29.243 9， 6.365 4-7.771 7， 37.327 4-45.671 2， 17.169 9-21.985 9， 66.940 4-91.206 3 mg/g， respectively. CONCLUSIONS The established method of component identification， fingerprint and content determination is stable， feasible and reliable， which is suitable for the comprehensive quality evaluation and control of EPDM.
