Relationship of chronic central pain and substance P in spinal dorsal horn after spinal cord injury

VernacularTitle:脊髓损伤后慢性中枢性疼痛与脊髓背角P物质关系研究
Author: Zhifang LIU ; Hong DAI ; Xiulai GAO ; Zhongxin XIAO ; Peng JING
Publication Type:Journal Article
Keywords: spinal cord injury (SCI), chronic central pain, spinal dorsal horn, substance P (SP), immunohistochemical assay, confocal laser scanning microscope
From: Chinese Journal of Rehabilitation Theory and Practice 2003;9(12):719-721
CountryChina
Language:Chinese
Abstract: Objective To approach the neurobiochemical mechanism of chronic central pain (CCP) after spinal cord injury (SCI). Methods 28 SD rats were divided into four groups, the normal group (group A), the pseudosurgery group (group B), and groups with CCP (group C) and without CCP (group D) after L1 spinal cord section injured with WADE method. T13 and L2 segments of rats' spinal cord were took and concentration changes of substance P (SP) in the spinal dorsal horn between two sections were examined by immunofluorescence histochemistry staining combined with confocal laser scanning microscope. Results Concentration of SP in the group D was decreased significantly compared with groups C,A and B (P<0.05-0.01), that of the group C was less than that of group A and B (P<0.05). Conclusion The rat model established by WADE method is proper to study CCP after SCI. SP in dorsal horn of spinal cord may inhibit the CCP after SCI in some degrees.