Protective effect of Acronychia pedunculata water extract on photoaging keratinocyte model and its mechanism
10.3760/cma.j.issn.1671-0290.2022.06.018
- VernacularTitle:降真香水提取物对光老化角质形成细胞模型的保护作用及其机制研究
- Author:
Xiuling LUO
1
;
Yuan ZHOU
;
Binbin ZHAO
;
Manli HUANG
;
Wenyu LI
;
Runge FAN
;
Sijian WEN
;
Jichao LI
;
Huayu WU
;
Youkun LIN
Author Information
1. 广西医科大学第一附属医院皮肤性病科，南宁 530022
- Keywords:
Acronychia pedunculatat;
Water extract;
Human keratinocytes;
Ultraviolet B;
Photoaging
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2022;28(6):517-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect and mechanism of Acronychia pedunculata water extracts on UV-induced light damage of human keratinocytes.Methods:The experiment was conducted from December 2018 to April 2020 in the Guangxi Medical University Laboratory of Genetics. The photoaged keratinocyte model was used, the cells were co-cultured with different concentrations of Acronychia pedunculata water extracts. The cell proliferation rate was detected by CCK-8 method. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total antioxidant capacity (T-AOC) of cells were detected by a test kit. The levels of IL-1β, IL-6 and tumor necrosis factor-alpha (TNF-α) were determined by ELISA.Results:The proliferation of HaCaT cells was promoted by 0.5 mg/L-2.0 mg/L of the extracts. Compared with control group, the proliferation rate of HaCaT cells in the experimental group was significantly increased ( P<0.05). Compared with control group, the contents of ROS was decreased ( F=214.67, P<0.05), MDA was decreased ( F=811.88, P<0.05), SOD was increased ( F=28.95, P<0.05), CAT was increased ( F=213.31, P<0.05), GPX was increased ( F=65.10, P<0.05), T-AOC was increased ( F=305.58, P<0.05), IL-1β was decreased ( F=15.46, P<0.05), IL-6 was decreased ( F=59.2, P<0.05), and TNF-α was decreased ( F=33.13, P<0.05). Conclusions:The extracts of 0.5-2.0 mg/L of Acronychia pedunculata have protective effects on the photoaging cell model, which may be related to the increase of SOD, CAT, GPX and other antioxidant enzymes and the level of T-AOC in photoaging HaCaT cells, and the decrease of ROS, MDA content and the expression of inflammatory cytokines.
